Results 131 to 140 of about 165,809 (296)

Multi-factor data normalization enables the detection of copy number aberrations in amplicon sequencing data

, 2014
International audienceAbstract Motivation: Because of its low cost, amplicon sequencing, also known as ultra-deep targeted sequencing, is now becoming widely used in oncology for detection of actionable mutations, i.e.
Gestraud, Pierre, Le Tourneau, Christophe, Boeva, Valentina, Barillot, Emmanuel, Rio Frio, Thomas, Toffoli, Sebastien, Servant, Nicolas, Popova, Tatiana, Hupé, Philippe, Lienard, Maxime, Gentien, David, Laes, Jean-François, Kamal, Maud   +12 more
core   +1 more source

Introgressed Variation in TaMYB7‐A1 Drives Graded Dormancy and Climate‐Adaptive Pre‐Harvest Sprouting Resistance in Wheat

Advanced Science, EarlyView.
TaMYB7‐A1 directly activates TaABI5 to enhance ABA signaling and regulate ABA‐GA homeostasis, enforcing seed dormancy. Its superior allele, derived from wild einkorn introgression, harbors a MITE insertion that elevates expression and two amino acid substitutions that enhance transcriptional activity, collectively generating graded PHS resistance for ...
Hao Wang, Dongzhi Wang, Yafei Guo, Qi Zheng, Min Zhang, James Simmonds, Jian Hou, Xuemei Liu, Xuelei Lin, Xiaomin Bie, Xiansheng Zhang, Xiaohui Li, Yuan Chen, Xueyong Zhang, Cristobal Uauy, Fei Lu, Chengcai Chu, Zhixi Tian, Jun Xiao   +18 more
wiley   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Deciphering intra-species bacterial diversity using gyrB amplicon sequencing [PDF]

, 2019
Présentation le 4 juillet 2019Deciphering intra-species bacterial diversity using [i]gyrB[/i] amplicon sequencing. JOBIM 2019.
Rué, Olivier
core  

Cotton Recruits Soil‐Derived Delftia tsuruhatensis to Suppress Aphid Detoxification Via Salicylic Acid‐Mediated Defense

Advanced Science, EarlyView.
This study systematically reveals a complex interactive network involving plants, microbes, and insects, elucidating the ecological and molecular mechanisms by which cotton enhances its resistance to aphids through the active recruitment of the beneficial soil bacterium Delftia tsuruhatensis.
Hui Xue, Fengchao Diao, Qiqing Yu, Xiangzhen Zhu, Tingfang Zhong, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Junyu Luo, Jinjie Cui, Xueke Gao   +11 more
wiley   +1 more source

Bumble bees used in amplicon sequencing and ARISA, and their geographic origins.

, 2015
For amplicon sequencing, bumble bees whose DNAs were pooled prior to Roche 454 library preparation were given the same pyrosequencing sample ID.Bumble bees used in amplicon sequencing and ARISA, and their geographic origins.
Haw Chuan Lim (701952), Manfredo J. Seufferheld (214812), Sydney A. Cameron (637454), Chia-Ching Chu (701953)   +3 more
core   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

An amplicon-based sequencing approach for Usutu virus characterization

Virology Journal
AbstractUsutu virus (USUV), an arbovirus from the Flaviviridae family, genus Flavivirus, has recently gained increasing attention because of its potential for emergence. After his discovery in South Africa, USUV spread to other African countries, then emerged in Europe where it was responsible for epizootics.
Ndione, Marie Henriette Dior, Diagne, Moussa Moïse, Mencattelli, Giulia, Diallo, Amadou, Ndiaye, El Hadji, Di Domenico, Marco, Diallo, Diawo, Kane, Mouhamed, Curini, Valentina, Top, Ndeye Marieme, Marcacci, Maurilia, Sankhe, Safiétou, Ancora, Massimo, Secondini, Barbara, Di Lollo, Valeria, Teodori, Liana, Leone, Alessandra, Puglia, Ilaria, Gaye, Alioune, Sall, Amadou Alpha, Loucoubar, Cheikh, Rosà, Roberto, Diallo, Mawlouth, Monaco, Federica, Faye, Ousmane, Cammà, Cesare, Rizzoli, Annapaola, Savini, Giovanni, Faye, Oumar   +28 more
openaire   +3 more sources

Xenogeneic Mitochondrial Transplantation Improves Selected Age‐Associated Phenotypes in Mice

Advanced Science, EarlyView.
Yak‐derived xenogeneic mitochondrial transplantation improves selected age‐associated phenotypes in mice, enhances mitochondrial functional readouts, and engages host mitochondrial quality‐control pathways. Broad tissue biodistribution, increased ATP production and mtDNA copy number, reduced ROS levels and dysfunctional mitochondria, improved motility ...
Wenpeng Li, Xijia Wang, Yaning Hu, Zhen Yang, Tian Niu, Xingbo Zhao   +5 more
wiley   +1 more source

MOESM9 of A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing

, 2019
Additional file 9: Table S8. Primer sequences for targeted amplicon sequencing.
Adeline Sourdille (7307531), Abdellah Barakate (7307528), Robbie Waugh (255169), Nicola Uzrek (6533999), Luke Ramsay (255158), Malcolm Macaulay (146819), Miriam Schreiber (529700), Pete Hedley (4641694), Jenny Morris (255155)   +8 more
core   +1 more source