Results 41 to 50 of about 371,602 (264)

Engineering a non-oxidative glycolysis pathway in escherichia coli for high-level citramalate production

Microbial Cell Factories
Background Methyl methacrylate (MMA) is a key precursor of polymethyl methacrylate, extensively used as a transparent thermoplastic in various industries.
Tingting Wang, Lijuan Ding, Huiying Luo, Huoqing Huang, Xiaoyun Su, Yingguo Bai, Tao Tu, Yuan Wang, Xing Qin, Honglian Zhang, Yaru Wang, Bin Yao, Jie Zhang, Xiaolu Wang   +13 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Mechanism of a polyherbal mixture alleviates calf diarrhea: an integrated network pharmacology, metabolomics, and microbiome study

Journal of Animal Science and Biotechnology
Background Calf diarrhea represents a prevalent and serious challenge in dairy farming. While medicinal plants demonstrate considerable potential for preventing calf diarrhea within antibiotic-free farming systems, their active components and mechanisms ...
Shaoxiong Ji, Jianmin Xia, Zhantao Yang, Siyuan Liu, Xinyue Zhang, Xiaojing Liu, Yangyi Hao, Wei Wang, Shenfei Long, Shengli Li   +9 more
doaj   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Lonicerae flos and turmeric extracts alleviate necrotic enteritis in broilers by modulating gut-liver health and microbiota

Journal of Animal Science and Biotechnology
Background Necrotic enteritis (NE) can cause intestinal barrier dysfunction in broilers, leading to secondary liver injury (SLI). In this process, the gut-liver axis plays a crucial role. Lonicerae flos and turmeric extracts (LTE), containing chlorogenic
Xingbo Liu, Yunru Ji, Huiyuan Lv, Zhong Wang, Zengpeng Lv, Yuming Guo, Wei Nie   +6 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Pooled feces reduce the inter-sample variation of microbiota in pig models

BMC Microbiology
Background For in vitro batch fermentation or fecal microbiota transplantation, preparation of inoculum was recommended by pooled feces to minimize inter-individual variation. However, the impact of pooling on microbiota variability remains unclear.
Huixin Wang, Qingtao Gao, Guoqi Dang, Shunfen Zhang, Ruqing Zhong, Liang Chen, Hongfu Zhang   +6 more
doaj   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Exploring the predominant factors associated with shaping the cultivable swine gut microbiota of Wuzhishan piglets under in vitro cultivation conditions

BMC Microbiology
Background Recent advances in swine gut culturomics have substantially expanded our understanding of the cultivable repertoire and revealed the compositional complexity of the porcine gut microbiota.
Yinfeng Chen, Xin Zhang, Xiaozhe Wang, Zhenlong Wu   +3 more
doaj   +1 more source