Results 51 to 60 of about 21,434 (259)

Feasibility of in vivo small animal imaging using a clinical total-body PET/CT system

EJNMMI Physics
Background Clinical PET scanners have long been explored for preclinical imaging, but limited spatial resolution and sensitivity have restricted their use for preclinical studies.
Julia G. Mannheim, Wenhong Lan, Maurizio Conti, Franziska Siedler, Marcel A. Krueger, Kristina Herfert, Christian la Fougère, Fabian P. Schmidt   +7 more
doaj   +1 more source

Extracellular matrix remodeling and immune reprogramming drive residual tumor progression of liver cancer after incomplete microwave ablation

Molecular Oncology, EarlyView.
Incomplete microwave ablation (iMWA) of liver cancer triggers a biphasic progression in residual tumors. At Day 3, the microenvironment is characterized by acute inflammatory responses and extracellular matrix (ECM) remodeling. By Day 14, a profound shift occurs toward oncogenic signal transduction and immunosuppression, marked by macrophage ...
Yu Liu, Yubei He, Nikolaus Berndt, Michael Mülleder, Kathrin Textoris‐Taube, Jing Guo, Matthias Stechele, Justus Ramtke, Shraga Nahum Goldberg, Lynn Jeanette Savic   +9 more
wiley   +1 more source

Protocols for Dual Tracer PET/SPECT Preclinical Imaging

Frontiers in Physics, 2020
Background: Multi-tracer PET/SPECT imaging enables different modality tracers to be present simultaneously, allowing multiple physiological processes to be imaged in the same subject, within a short time-frame.
Julia E. Blower, Jayanta K. Bordoloi, Alex Rigby, Matthew Farleigh, Jana Kim, Hugh O'Brien, Jonathan Jackson, Constantinos Poyiatzis, James Bezer, Kavitha Sunassee, Philip J. Blower, Lefteris Livieratos   +11 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Comparison of scandium-44 g with other PET radionuclides in pre-clinical PET phantom imaging

EJNMMI Physics, 2019
Purpose The decay characteristics of radionuclides in PET studies can impact image reconstruction. 44gSc has been the topic of recent research due to potential theranostic applications and is a promising radiometal for PET imaging.
Simon Ferguson, Hans-Sonke Jans, Melinda Wuest, Terence Riauka, Frank Wuest   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Feasibility Study of In-Beam PET Imaging for Dose Verification in Carbon Therapy Using Free PET

Journal of Isotopes, 2022
In-beam PET imaging provides a noninvasive method to monitor the dose distribution in real-time for particle therapy such as carbon ion therapy and proton therapy.
PEI Changxu;WEN Jing;LIU Meilou;GUO Dian;ZHANG Qinghua;HUANG Chuan;LI Yingguo;YIN Yongzhi;CHEN Ximeng
doaj  

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Activity painting: PET images of freely defined activity distributions applying a novel phantom technique.

PLoS ONE, 2019
The aim of this work was to develop a novel phantom that supports the construction of highly reproducible phantoms with arbitrary activity distributions for PET imaging. It could offer a methodology for answering questions related to texture measurements
Attila Forgacs, Piroska Kallos-Balogh, Ferenc Nagy, Aron K Krizsan, Ildiko Garai, Lajos Tron, Magnus Dahlbom, Laszlo Balkay   +7 more
doaj   +1 more source