Results 281 to 290 of about 1,768,583 (320)
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The use of concanavalin A for distinguishing IgM from IgG antibody-producing cells.
Journal of Immunology, 1969Harris and Robson (1) have reported the ability of concanavalin A to precipitate several classes of serum glycoproteins. This selective precipitation is probably due to the specificity of concanavalin A for non-reducing mannosyl and N ...
Albert A. Nordin +2 more
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Immunocytochemical localization of antibody-producing cells in adult lamprey
Immunology Letters, 1983Antibody-producing cells were localized in the fat column of adult lamprey previously immunized with 'O'RBC. These cells were visualized using rabbit anti-lamprey antibody antiserum in the peroxidase anti-peroxidase (PAP) immunocytochemical technique.
Michael F. Filosa +2 more
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Analysis of Immunotoxicity by Enumeration of Antibody‐Producing B Cells
Current Protocols in Toxicology, 2006AbstractThe plaque‐forming cell (PFC) assay measures the humoral immune response mediated by the concerted actions of antigen‐presenting cells, T lymphocytes, and B lymphocytes. The most common form of the plaque method is used for the detection of murine primary IgM antibodies directed against the T cell–dependent sheep red blood cell (sRBC) surface ...
Albert E. Munson +2 more
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Generation of monoclonal antibody-producing mammalian cell lines [PDF]
Pharmaceutical Bioprocessing, 2013 Clinical effectiveness has driven the commercial success of monoclonal antibody (mAb) products. Mammalian cells are currently the preferred system for large-scale production as the mAbs produced are biochemically similar to human forms. The cell line generation process is tedious and time-consuming as clones with high productivity, stable long-term ...
Steven CL Ho +2 more
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SERUM FREE CRYOPRESERVATION OF ANTIBODY PRODUCING CELLS
1992ABSTRACT The use of Foetal Bovine Serum (FBS) in the culture of cells for the production of human therapeutics is undesirable. The risk of introducing mycoplasma, viruses, entities responsible for spongiform encephalopathy and other adventitious agents from FBS can be eliminated in culture by the use of serum free media.
R. Chung +4 more
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Separation of mitogen-induced suppressor cells of human antibody-producing cells
Cellular Immunology, 1980Abstract Human antibody-forming cells were demonstrated by a plaque in agar technique following in vitro stimulation with either pokeweed mitogen or Cowan I strain of protein A-positive Staphylococcus aureus bacteria. We evaluated the effects on this antibody formation caused by the addition of cells which had been stimulated with PH A or Con A. Both
Hans Wigzell +2 more
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Growth of antibody-producing cell clones in microcultures
1987Publisher Summary This chapter describes growth of antibody-producing cell clones in microcultures. The objective of the microculture technique is to analyze the frequency of rare cells involved in the immune response. The precursors of antibody-forming cells (B cells), helper T cells, and suppressor T cells are considered rare cells.
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Effect of antibiotics on the development of antibody-producing cells, in vitro
Biochemical Pharmacology, 1972Abstract Using the Mishell-Dutton technique † for culturing mouse spleen cells to produce hemolysin plaque-forming cells (PFC) in vitro , actinomycin D, puromycin or cycloheximide were added daily to determine antibiotic-sensitive sites. The culture period was functionally divided into three temporal phases: (a) An initial phase (0–24 hr) of ...
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Low number of antibody producing cells in patients with sickle cell anemia
Immunology Letters, 1992B cell function is impaired in patients with sickle cell anemia. Although the number of surface IgM positive cells was similar in sickle cell patients and controls, in vitro spontaneous IgM, and PWM stimulated IgA, IgM, and IgG synthesis was significantly lower in the patients than in controls.
Natasha L. Martin +5 more
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Toxin–antigen conjugates as selection tools for antibody producing cells
Journal of Immunological Methods, 2013The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure.
Messerschmidt, Katrin, Heilmann, Katja
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