Results 41 to 50 of about 89,614 (291)

Separating clinical antibodies from repertoire antibodies, a path to in silico developability assessment

mAbs, 2022
Approaches for antibody discovery have seen substantial improvement and success in recent years. Yet, advancing antibodies into the clinic remains difficult because therapeutic developability concerns are challenging to predict.
Christopher Negron, Joyce Fang, Michael J. McPherson, W. Blaine Stine, Andrew J. McCluskey   +4 more
doaj   +1 more source

High throughput long-read sequencing of circulating lymphocytes of the evolutionarily distant sea lamprey reveals diversity and common elements of the variable lymphocyte receptor B (VLRB) repertoire

Frontiers in Immunology
The leucine-rich repeat-based variable lymphocyte receptor B (VLRB) antibody system of jawless vertebrates is capable of generating an antibody repertoire equal to or exceeding the diversity of antibody repertoires of jawed vertebrates.
Amin Zia, Ariel Orozco, Irene S. Y. Fang, Aspen M. Tang, Ana Sofia Mendoza Viruega, Shilan Dong, Leslie Y. T. Leung, Vijaya M. Devraj, Opeyemi E. Oludada, Götz R. A. Ehrhardt   +9 more
doaj   +1 more source

Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors

Frontiers in Immunology, 2018
Age-related changes can significantly alter the state of adaptive immune system and often lead to attenuated response to novel pathogens and vaccination.
Alexey N. Davydov, Anna S. Obraztsova, Anna S. Obraztsova, Mikhail Y. Lebedin, Maria A. Turchaninova, Maria A. Turchaninova, Maria A. Turchaninova, Dmitriy B. Staroverov, Dmitriy B. Staroverov, Ekaterina M. Merzlyak, Ekaterina M. Merzlyak, George V. Sharonov, George V. Sharonov, Olga Kladova, Mikhail Shugay, Mikhail Shugay, Mikhail Shugay, Mikhail Shugay, Olga V. Britanova, Olga V. Britanova, Olga V. Britanova, Dmitriy M. Chudakov, Dmitriy M. Chudakov, Dmitriy M. Chudakov, Dmitriy M. Chudakov, Dmitriy M. Chudakov   +25 more
doaj   +1 more source

Exploiting B Cell Receptor Analyses to Inform on HIV-1 Vaccination Strategies

Vaccines, 2020
The human antibody repertoire is generated by the recombination of different gene segments as well as by processes of somatic mutation. Together these mechanisms result in a tremendous diversity of antibodies that are able to combat various pathogens ...
Christoph Kreer, Henning Gruell, Thierry Mora, Aleksandra M. Walczak, Florian Klein   +4 more
doaj   +1 more source

A Customizable Suite of Methods to Sequence and Annotate Cattle Antibodies

Vaccines, 2023
Studying the antibody response to infection or vaccination is essential for developing more effective vaccines and therapeutics. Advances in high-throughput antibody sequencing technologies and immunoinformatic tools now allow the fast and comprehensive ...
Kristel Ramirez Valdez, Benjamin Nzau, Daniel Dorey-Robinson, Michael Jarman, James Nyagwange, John C. Schwartz, Graham Freimanis, Angela W. Steyn, George M. Warimwe, Liam J. Morrison, William Mwangi, Bryan Charleston, Marie Bonnet-Di Placido, John A. Hammond   +13 more
doaj   +1 more source

From rabbit antibody repertoires to rabbit monoclonal antibodies [PDF]

Experimental & Molecular Medicine, 2017
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and
Weber, Justus, Peng, Haiyong, Rader, Christoph   +2 more
openaire   +2 more sources

In-Depth Analysis of Human Neonatal and Adult IgM Antibody Repertoires

Frontiers in Immunology, 2018
Although high-throughput sequencing and associated bioinformatics technologies have enabled the in-depth, sequence-based characterization of human immune repertoires, only a few studies on a relatively small number of sequences explored the ...
Binbin Hong, Binbin Hong, Yanling Wu, Wei Li, Xun Wang, Yumei Wen, Shibo Jiang, Dimiter S. Dimitrov, Tianlei Ying   +8 more
doaj   +1 more source

Germline IGHV3-53-encoded RBD-targeting neutralizing antibodies are commonly present in the antibody repertoires of COVID-19 patients

Emerging Microbes and Infections, 2021
Monoclonal antibodies (mAbs) encoded by IGHV3-53 (VH3-53) targeting the spike receptor-binding domain (RBD) have been isolated from different COVID-19 patients.
Qihong Yan, Ping He, Xiaohan Huang, Kun Luo, Yudi Zhang, Haisu Yi, Qian Wang, Feng Li, Ruitian Hou, Xiaodi Fan, Pingchao Li, Xinglong Liu, Huan Liang, Yijun Deng, Zhaoming Chen, Yunfei Chen, Xiaoneng Mo, Liqiang Feng, Xiaoli Xiong, Song Li, Jian Han, Linbing Qu, Xuefeng Niu, Ling Chen   +23 more
doaj   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source