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The Antigen-Antibody Reaction in Immunohistochemistry [PDF]
The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in
exaly +3 more sources
Acute hemolytic transfusion reaction mediated by anti-Jkb antibody in a pediatric patient with β-thalassemia major: a case report illustrating an anamnestic alloimmune response [PDF]
Non-ABO alloantibody-mediated acute hemolytic transfusion reactions are infrequent but life-threatening complications of red blood cell transfusion. Anti-Jkb antibodies are difficult to detect in routine pretransfusion screening due to their low titers ...
Jingyuan Xu +5 more
doaj +2 more sources
Development of a polyclonal antibody Edwardsiella ictaluri serum for diagnosis of Enteric Septicemia of Catfish (ESC) in Patin Catfish (Pangasianodon hypopthalmus) [PDF]
Enteric Septicemia of Catfish (ESC), caused by Edwarsiella ictaluri, has been considered a major bacterial disease in catfish, including Patin (Pangasionodon hypophthalmus), causing economic losses.
Kholidin Edy Barkat +4 more
doaj +1 more source
Rare Anti-Cw antibody: Two case reports with review of literature
Introduction: Anti-Cw is a low-frequency antibody against Cw (Rh8) antigen, a low incidence antigen of the Rh system. Anti-Cw antibody may cause mild to moderate hemolytic disease of the fetus and newborn (HDFN) and mild to severe immediate or delayed ...
Sadhana Mangwana +2 more
doaj +1 more source
Polyclonal antibodies against potato spindle tuber viroid RNA
Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin +2 more
doaj +1 more source
BACKGROUND: Antigen-antibody reactions are a special field of molecular interactions. The physico-chemical nature of antigen-antibody binding and ligand-induced changes in the fine molecular structures of antigens during immunocomplex formation are less ...
Peter Nemeth
doaj +1 more source
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips [PDF]
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the ...
So-Woon Kim, Jin Roh, Chan-Sik Park
doaj +1 more source
VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform
Fluorescence-linked immunosorbent assay (FLISA) is a commonly used, quantitative technique for detecting biochemical changes based on antigen–antibody binding reactions using a well-plate platform.
Dong Hee Kang +3 more
doaj +1 more source
Photoreversible antigen—antibody reactions [PDF]
A monoclonal antibody (Z1H01) for an oligopeptide carrying an azobenzene group, was prepared under conditions where the azobenzene group is in the trans form. The antibody bound the hapten peptide effectively when the hapten peptide is in the trans form (K=5 × 107 M−1), but the antibody released the hapten under irradiation with UV light where the ...
Harada, Masataka +3 more
openaire +2 more sources
Gp.Mur is a clinically relevant antigen of the MNS blood group system that is highly prevalent in several Asian populations. Its corresponding antibody, anti-Gp.Mur, has been implicated in hemolytic transfusion reactions and hemolytic disease of the ...
Robert John S. Lamis +5 more
doaj +1 more source

