Transformation by Polyomavirus Middle T Antigen Involves a Unique Bimodal Interaction with the Hippo Effector YAP [PDF]
Journal of Virology, 2016 ABSTRACT Murine polyomavirus has repeatedly provided insights into tumorigenesis, revealing key control mechanisms such as tyrosine phosphorylation and phosphoinositide 3-kinase (PI3K) signaling. We recently demonstrated that polyomavirus small T antigen (ST) binds YAP, a major effector of Hippo signaling, to regulate differentiation. Here we
Cecile, Rouleau +7 more
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Domains in Middle-T Antigen That Cooperate in Polyomavirus-Mediated Oncogenic Transformation
Virology, 1995 Middle-T antigen is the oncogenic protein of Polyomavirus and associates with several cellular enzymes involved in signal transduction, e.g., Src tyrosine kinases, phosphatidylinositol 3-kinase (PI 3-kinase), protein phosphatase 2A (PP2A), and Shc, an SH2 domain-containing adapter protein. We have shown earlier that middle-T is a target of a cell cycle-
Pérez L +5 more
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The p110α Isoform of Phosphatidylinositol 3-Kinase Is Essential for Polyomavirus Middle T Antigen-Mediated Transformation [PDF]
Journal of Virology, 2007 ABSTRACT Middle T antigen (MT) of polyomavirus is known to play an important role in virus-mediated cellular transformation. While MT has been extensively examined in spontaneously immortalized rodent fibroblasts, its interactions with cells of other types and species are less well understood.
Tamara, Utermark +3 more
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Polyomavirus Middle T Antigen Induces the Transcription of Osteopontin, a Gene Important for the Migration of Transformed Cells [PDF]
Journal of Virology, 2008 ABSTRACT Middle T antigen (MT) is the principal oncoprotein of murine polyomavirus. Experiments on the acute immediate effects of MT expression on cellular RNA levels showed that expression of osteopontin (OPN) was strongly induced by MT expression. Osteopontin is a protein known to be associated with cancer.
Kerry A, Whalen +3 more
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Transformation and tumorigenic properties of a mutant polyomavirus containing a middle T antigen defective in Shc binding [PDF]
Journal of Virology, 1997 Polyomavirus middle T antigen is phosphorylated on several tyrosine residues which act as binding sites for cellular proteins, including phosphatidylinositol 3-kinase, Shc, and phospholipase C-gamma. In this report we describe the transforming properties and tumor-inducing ability of a polyomavirus that contains a single-site mutation in middle T ...
X, Yi, J, Peterson, R, Freund
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Cooperation of middle and small T antigens of polyomavirus in transformation of established fibroblast and epithelial-like cell lines [PDF]
Journal of Virology, 1987 We have reported recently that small T antigen of polyomavirus stimulates the growth of NIH 3T3 cells beyond their saturation density and induces weak anchorage-independent growth (T. Noda, M. Satake, T. Robins, and Y. Ito, J. Virol. 60:105-113, 1986).
T, Noda, M, Satake, Y, Yamaguchi, Y, Ito
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Analysis of polyomavirus middle-T-antigen-transformed rat cell variants expressing different levels of pp60c-src [PDF]
Journal of Virology, 1987 We characterize two independent variant cellular clones which arose following in vitro passage of polyomavirus middle-T-antigen (MTAg)-transformed FR3T3 cells expressing RNA complementary to c-src mRNA. These clones were initially flat and underwent morphologic transformation at a high frequency to a phenotype indistinguishable from that of parental ...
J B, Bolen +4 more
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Transformation of enriched mammary cell populations with polyomavirus middle T antigen influences tumor subtype and metastatic potential [PDF]
Breast Cancer Research, 2015 Breast cancer exhibits significant molecular, histological, and pathological diversity. Factors that impact this heterogeneity are poorly understood; however, transformation of distinct normal cell populations of the breast may generate different tumor phenotypes.
Drobysheva, Daria +5 more
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Medium tumor antigen of polyomavirus transformation-defective mutant NG59 is associated with 73-kilodalton heat shock protein [PDF]
Journal of Virology, 1987 Affinity-purified medium T antigen encoded by NG59, a nontransforming mutant of polyomavirus, is specifically associated with a protein of 72,000 daltons (72K protein). Medium T antigens of wild-type polyomavirus and the transformation-competent mutant dl8 are not associated with the 72K protein.
G, Walter, A, Carbone, W J, Welch
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Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector. [PDF]
Molecular and Cellular Biology, 1986 The middle T antigen of polyomavirus transformed primary chicken embryo fibroblasts when expressed from a replication-competent avian retrovirus. This in vitro-constructed retrovirus, SRMT1, is a variant of Rous sarcoma virus that encodes the middle T antigen in place of v-src.
S, Kornbluth +3 more
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