Results 51 to 60 of about 93,358 (167)

Repair of naturally occurring mismatches can induce mutations in flanking DNA

eLife, 2014
‘Normal’ genomic DNA contains hundreds of mismatches that are generated daily by the spontaneous deamination of C (U/G) and methyl-C (T/G). Thus, a mutagenic effect of their repair could constitute a serious genetic burden.
Jia Chen, Brendan F Miller, Anthony V Furano   +2 more
doaj   +1 more source

FHIT loss-induced DNA damage creates optimal APOBEC substrates: Insights into APOBEC-mediated mutagenesis

Oncotarget, 2014
APOBEC cytidine deaminase activity is a major source of hypermutation in cancer. But previous studies have shown that the TC context signature of these enzymes is not observed in sizable fractions of cancers with overexpression of APOBEC, suggesting that cooperating factors that contribute to this mutagenesis should be identified. The fragile histidine
Catherine E, Waters, Joshua C, Saldivar, Zaynab A, Amin, Morgan S, Schrock, Kay, Huebner   +4 more
openaire   +3 more sources

Pan-Cancer Survey of Tumor Mass Dormancy and Underlying Mutational Processes

Frontiers in Cell and Developmental Biology, 2021
Tumor mass dormancy is the key intermediate step between immune surveillance and cancer progression, yet due to its transitory nature it has been difficult to capture and characterize. Little is understood of its prevalence across cancer types and of the
Anna Julia Wiecek, Daniel Hadar Jacobson, Daniel Hadar Jacobson, Wojciech Lason, Maria Secrier   +4 more
doaj   +1 more source

APOBEC in breast cancer: a dual player in tumor evolution and therapeutic response

Frontiers in Molecular Biosciences
The APOBEC (Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like) family of cytidine deaminases has emerged as pivotal a contributor to genomic instability and adaptive immunity through DNA/RNA editing. Accumulating evidence underscores their
Haiqi Lu, Zelin Lu, Yufei Wang, Miaoqin Chen, Guangliang Li, Xian Wang   +5 more
doaj   +1 more source

Human Papillomavirus Carcinogenesis Mediated by APOBEC Mutagenesis

YAKUGAKU ZASSHI, 2019
Persistent infection with oncogenic human papillomaviruses (HPVs) is necessary for the development of cervical cancer, although the accumulation of somatic mutations in the host genome is also required for the generation of invasive cervical cancer. Recent studies have demonstrated concomitant genetic changes in the HPV genome; however, their relevance
openaire   +3 more sources

APOBEC3A and APOBEC3B Preferentially Deaminate the Lagging Strand Template during DNA Replication

Cell Reports, 2016
APOBEC family cytidine deaminases have recently been implicated as powerful mutators of cancer genomes. How APOBECs, which are ssDNA-specific enzymes, gain access to chromosomal DNA is unclear. To ascertain the chromosomal ssDNA substrates of the APOBECs,
James I. Hoopes, Luis M. Cortez, Tony M. Mertz, Ewa P. Malc, Piotr A. Mieczkowski, Steven A. Roberts   +5 more
doaj   +1 more source

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Correlation of gene expression and associated mutation profiles of APOBEC3A, APOBEC3B, REV1, UNG, and FHIT with chemosensitivity of cancer cell lines to drug treatment

Human Genomics, 2018
Background The APOBEC gene family of cytidine deaminases plays important roles in DNA repair and mRNA editing. In many cancers, APOBEC3B increases the mutation load, generating clusters of closely spaced, single-strand-specific DNA substitutions with a ...
Suleyman Vural, Richard Simon, Julia Krushkal   +2 more
doaj   +1 more source

Deciphering transcriptome complexity via long‐read sequencing

iMeta, EarlyView.
Long‐read sequencing is transforming transcriptomics from gene‐level quantification to isoform‐resolved interpretation by directly resolving full‐length transcript structures, transcription in repetitive regions, and linked molecular features. This review provides an end‐to‐end roadmap covering sequencing platforms, library preparation strategies ...
Chuwen Xu, Jia Li, Chenxi Yin, Keying Li, Tian Wang, Shiwen Gao, Yue Yu, Cheng Chang, Ye Wang, Guoqing Tong, Liang Gong, Wen Hu, Guoliang Chai, Yilai Han, Qian Qin, Suoqin Jin, Xiufen Zou, Bo Li, Tianyuan Zhang, Ana Conesa, Yunhao Wang, Dingjie Wang   +21 more
wiley   +1 more source

Abstract 1292: Germline genetic, molecular and environmental factors modulate APOBEC mutagenesis in human tumors

, 2017
Background: Activity of APOBEC3 enzymes has been identified as a main source of somatic mutations of a specific type (C to T or G substitutions in the TCA or TCT motifs).
Ludmila Prokunina-Olsson, Ariunaa Bayanjargal, Olusegun O Onabajo, A. Rouf Banday, Krizia-Ivana Udquim   +4 more
core   +1 more source