Prime Editing: Mechanistic Insights and DNA Repair Modulation
CellsPrime editing is a genome editing technique that allows precise modifications of cellular DNA without relying on donor DNA templates. Recently, several different prime editor proteins have been published in the literature, relying on single- or double ...
Astrid Mentani +2 more
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Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems
Nature CommunicationsPrime editing is a genome engineering tool that allows installation of various small edits with high precision. However, prime editing efficiency and purity can vary widely across different edits, genomic targets, and cell types.
Louis C. Dacquay +12 more
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mBio, 2015 The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, an RNA-guided nuclease for specific genome editing in vivo, has been adopted in a wide variety of organisms.
Yunkun Liu +6 more
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Grass Meal Acts as a Probiotic in Chicken
Microbiology Research, 2023 Probiotics can act as an alternative to antibiotics in animal feeding, but their use is minimal due to their expensive production. Dry grass is rich with bacteria beneficial for animal feeding and can be used as a probiotic.
Elena S. Bogdanova +7 more
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The origin of unwanted editing byproducts in gene editing
Acta Biochimica et Biophysica Sinica, 2022 The rapid development of CRISPR-Cas genome editing tools has greatly changed the way to conduct research and holds tremendous promise for clinical applications.
Yin Jianhang, Hu Jiazhi
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Rapid genome editing by CRISPR-Cas9-POLD3 fusion
eLife, 2021 Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are ...
Ganna Reint +18 more
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Examination of the Cell Cycle Dependence of Cytosine and Adenine Base Editors
Frontiers in Genome Editing, 2022 Base editors (BEs) are genome editing agents that install point mutations with high efficiency and specificity. Due to their reliance on uracil and inosine DNA damage intermediates (rather than double-strand DNA breaks, or DSBs), it has been hypothesized
Cameron A. Burnett +6 more
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Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases
Biomolecules, 2022 In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates.
Wataru Aiba +3 more
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No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells. [PDF]
PLoS ONE, 2017 The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells.
Parisa Javidi-Parsijani +5 more
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AID/APOBEC–dependent somatic hypermutation and DNA rearrangements of immunoglobulin and non-immunoglobulin genes [PDF]
Annals of Mechnikov's Institute, 2019 Editing Ig genes by activation induced deaminase (AID) initiates the antibody diversification process in B lymphocytes. In mammalian B cells, this process includes somatic hypermutation (SHM) and class switch recombination (CSR).
Kolotova T. +3 more
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