Results 21 to 30 of about 2,368,060 (250)

Chimeric oligonucleotides combining guide RNA and single-stranded DNA repair template effectively induce precision gene editing

RNA Biology, 2022
The ability to precisely alter the genome holds immense potential for molecular biology, medicine and biotechnology. The development of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) into a genomic editing tool has vastly ...
Avantika Ghosh, Ksenia Myacheva, Marisa Riester, Carla Schmidt, Sven Diederichs   +4 more
doaj   +1 more source

Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime Editor human induced pluripotent stem cell line

Stem Cell Research, 2021
Prime editing uses the Cas9 nickase fused to a reverse transcriptase to copy a DNA sequence into a specific locus from a ‘prime editing’ guide RNA (pegRNA), eliminating the need for double-stranded DNA breaks and donor DNA templates.
Nike Bharucha, Jennifer Arthur Ataam, Alexandra A. Gavidia, Ioannis Karakikes   +3 more
doaj   +1 more source

Amphioxus adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U and A-to-I deamination of DNA

Communications Biology, 2023
Adenosine-to-inosine tRNA-editing enzyme has been identified for more than two decades, but the study on its DNA editing activity is rather scarce. We show that amphioxus (Branchiostoma japonicum) ADAT2 (BjADAT2) contains the active site ‘HxE-PCxxC’ and ...
Zhan Gao, Wanyue Jiang, Yu Zhang, Liping Zhang, Mengmeng Yi, Haitao Wang, Zengyu Ma, Baozhen Qu, Xiaohan Ji, Hongan Long, Shicui Zhang   +10 more
doaj   +1 more source

Explore the dominant factor in prime editing via a view of DNA processing

Synthetic and Systems Biotechnology, 2023
Prime editing is a revolutionary gene-editing method that is capable of introducing insertions, deletions and base substitutions into the genome. However, the editing efficiency of Prime Editor (PE) is limited by the DNA repair process.
Zhimeng Xu, Dacheng Ma, Houzhen Su, Xiaodong Jia, Yinqing Li, Yinying Lu, Zhen Xie   +6 more
doaj   +1 more source

Editosome Accessory Factors KREPB9 and KREPB10 in Trypanosoma brucei [PDF]

, 2012
Multiprotein complexes, called editosomes, catalyze the uridine insertion and deletion RNA editing that forms translatable mitochondrial mRNAs in kinetoplastid parasites.
Acestor, Nathalie, Schnaufer, Achim; id_orcid, Stuart, Kenneth, Carnes, Jason, Lerch, Melissa, Guo, Xuemin   +5 more
core   +1 more source

Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-β signaling

Molecular Therapy: Nucleic Acids, 2022
Strategies to modulate cellular DNA repair pathways hold immense potential to enhance the efficiency of CRISPR-Cas9 genome editing platform. In the absence of a repair template, CRISPR-Cas9-induced DNA double-strand breaks are repaired by the endogenous ...
Tarun Mishra, Vipin Bhardwaj, Neha Ahuja, Pallavi Gadgil, Pavitra Ramdas, Sanjeev Shukla, Ajit Chande   +6 more
doaj   +1 more source

An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei [PDF]

, 2007
RNA editing in the sleeping sickness parasite Trypanosoma brucei remodels mitochondrial transcripts by the addition and deletion of uridylates as specified by guide RNAs.
Schnaufer, Achim; id_orcid, Stuart, Kenneth, Ernst, Nancy Lewis, Fleck, Michele, Babbarwal, Vinod Kumar   +4 more
core   +1 more source

DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements

BMC Biology, 2023
Background Recent studies have shown that, owning to its cohesive cleavage, Cas9-mediated CRISPR gene editing outcomes at junctions of chromosomal rearrangements or DNA-fragment editing are precise and predictable; however, the underlying mechanisms are ...
Mohammadreza M. Mehryar, Xin Shi, Jingwei Li, Qiang Wu   +3 more
doaj   +1 more source

Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing

Nature Communications, 2023
Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration and generation of unintended mutations ...
Sandra Wimberger, Nina Akrap, Mike Firth, Johan Brengdahl, Susanna Engberg, Marie K. Schwinn, Michael R. Slater, Anders Lundin, Pei-Pei Hsieh, Songyuan Li, Silvia Cerboni, Jonathan Sumner, Burcu Bestas, Bastian Schiffthaler, Björn Magnusson, Silvio Di Castro, Preeti Iyer, Mohammad Bohlooly-Y, Thomas Machleidt, Steve Rees, Ola Engkvist, Tyrell Norris, Elaine B. Cadogan, Josep V. Forment, Saša Šviković, Pinar Akcakaya, Amir Taheri-Ghahfarokhi, Marcello Maresca   +27 more
doaj   +1 more source

Identification of novel components of Trypanosoma brucei editosomes [PDF]

, 2003
The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Schnaufer, Achim; id_orcid, Wang, Bingbing, Salavati, Reza, Stuart, Kenneth, Carmean, Nicole, Panigrahi, Aswini K, Ernst, Nancy L   +6 more
core   +1 more source