Results 31 to 40 of about 2,368,060 (250)
Frame-Based Editing: Combining the Best of Blocks and Text Programming [PDF]
, 2016 Editing program code as text has several major weaknesses: syntax errors (such as mismatched braces) interrupt programmer flow and make automated tool support harder, boilerplate code templates have to be typed out, and programmers are responsible for ...
Neil C. C. Brown +7 more
core +1 more source
Non-Homologous End Joining and Homology Directed DNA Repair Frequency of Double-Stranded Breaks Introduced by Genome Editing Reagents. [PDF]
PLoS ONE, 2017 Genome editing using transcription-activator like effector nucleases or RNA guided nucleases allows one to precisely engineer desired changes within a given target sequence.
Michail Zaboikin +3 more
doaj +1 more source
The p53 challenge of hematopoietic stem cell gene editing
Molecular Therapy: Methods & Clinical Development, 2023 Ex vivo gene editing in hematopoietic stem and progenitor cells (HSPCs) represents a promising curative treatment strategy for monogenic blood disorders.
Sofie R. Dorset, Rasmus O. Bak
doaj +1 more source
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
Cell Reports, 2020 Summary: The advent of base editors (BEs) holds great potential for correcting pathogenic-related point mutations to treat relevant diseases. However, Cas9 nickase (nCas9)-derived BEs lead to DNA double-strand breaks, which can trigger unwanted DNA ...
Xiao Wang +17 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
, 2023 The advancement of precision engineering for crop trait improvement is important in the face of rapid population growth, climate change, and disease. To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for
Myeong-Je Cho (4031018) +8 more
core +1 more source
Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. [PDF]
PLoS ONE, 2018 Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the ...
Henriette O'Geen +4 more
doaj +1 more source
The human gut microbiome across the life course
FEBS Letters, EarlyView.Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero +4 more
wiley +1 more source
, 2023 The advancement of precision engineering for crop trait improvement is important in the face of rapid population growth, climate change, and disease. To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for
Myeong-Je Cho (4031018) +8 more
core +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source