Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
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DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection [PDF]
Frontiers in Genome EditingProtoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes.
Ryan Creeth +2 more
doaj +2 more sources
Developing Collaborative XML Editing Systems [PDF]
, 2007 In many areas the eXtensible Mark-up Language (XML) is becoming the standard exchange and data format. More and more applications not only support XML as an exchange format but also use it as their data model or default file format for graphic, text and ...
Gerlicher, Ansgar Robert Sandy
core +5 more sources
A novel genome editing technique in Chlamydomonas reinhardtii and its underlying DNA repair pathway [PDF]
, 2022 The green microalga Chlamydomonas reinhardtii is an important model organism, with potential industrial biotechnology applications, including potential for vaccine production (e.g., TransAlgae, Israel) and vegan protein (e.g., Triton Algae ...
Ferenczi, Aron
core +1 more source
Tissue Specific DNA Repair Outcomes Shape the Landscape of Genome Editing
Frontiers in Genetics, 2021 The use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 has moved from bench to bedside in less than 10years, realising the vision of correcting disease through genome editing. The accuracy and safety of this approach relies on
Mathilde Meyenberg +5 more
doaj +1 more source
Translational potential of base-editing tools for gene therapy of monogenic diseases
Frontiers in Bioengineering and Biotechnology, 2022 Millions of people worldwide have rare genetic diseases that are caused by various mutations in DNA sequence. Classic treatments of rare genetic diseases are often ineffective, and therefore great hopes are placed on gene-editing methods.
Vasiliy V. Reshetnikov +7 more
doaj +1 more source
Alternative splicing and extensive RNA editing of human TPH2 transcripts. [PDF]
, 2010 Brain serotonin (5-HT) neurotransmission plays a key role in the regulation of mood and has been implicated in a variety of neuropsychiatric conditions. Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of 5-HT.
Bondy, Brigitta +59 more
core +1 more source
Zinc finger recombinases with adaptable DNA sequence specificity [PDF]
, 2011 Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Proudfoot, C. +19 more
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Two steps are better than one: improving gene editing to treat cancer
FEBS Open Bio, 2022 Gene editing enables scientists to make precise changes to the genome of an organism using the cell’s own ability to repair damaged DNA using a supplied DNA template.
Francesca Minter
doaj +1 more source
DNA Damage Response Pathway and Replication Fork Stress During Oligonucleotide Directed Gene Editing
Molecular Therapy: Nucleic Acids, 2012 Single-stranded DNA oligonucleotides (ODNs) can be used to direct the exchange of nucleotides in the genome of mammalian cells in a process known as gene editing.
Melissa Bonner +4 more
doaj +1 more source