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Culture of shoot tips from adventitious shoots can eradicate Apple stem pitting virus but fails in Apple stem grooving virus

Plant Cell, Tissue and Organ Culture, 2016
This study attempted to eradicate Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) from ‘Gala’ apple using shoot tips from of adventitious shoots derived from leaf segments. Leaf segments were excised from in vitro 4-week-old stock shoots and cultured on shoot regeneration medium containing 9.1 µM thidiazuron and 0.25 µM indole-3 ...
Min-Rui Wang   +2 more
exaly   +2 more sources

Apple stem pitting virus. [Distribution map].

Distribution Maps of Plant Diseases, 2012
Abstract A new distribution map is provided for Apple stem pitting virus. Flexiviridae: Foveavirus. Hosts: Apple ( Malus domestica ), European pear ( Pyrus communis ) and stone fruit ( Prunus spp.).
openaire   +1 more source

RNA Secondary Structure and Sequence Variability of Apple Stem Pitting Virus

2009 2nd International Conference on Biomedical Engineering and Informatics, 2009
Nucleotide sequences of coat protein (CP) gene from 6 isolates of Apple stem pitting virus (ASPV) were obtained. Sequence analysis of CP gene showed nucleotide identities ranged from 68.7 to 99.7%. Phylogenetic analysis showed that all ASPV isolates fell into three groups. To a certain extent, the groups were related to the host origins of virus.
Li-li Li   +5 more
exaly   +2 more sources

Occurrence and genetic diversity analysis of apple stem pitting virus isolated from apples in China

Archives of Virology, 2017
Two primer pairs were used to detect apple stem pitting virus (ASPV) using a reverse transcription (RT)-PCR test. 82 out of the 141 randomly collected samples, from ten orchards in five provinces and regions of China, tested positive. In the positive samples forty-five (55%) were infected by ASPV and two other viruses.
Guo-Jun Hu   +5 more
openaire   +2 more sources

FIRST REPORT OF APPLE STEM PITTING VIRUS IN SHAANXI, CHINA

Journal of Plant Pathology, 2012
Apple is a major crop in the Shaanxi province of China. In this area, the decline of apple trees may be due to infection by viruses and/or other pathogens that cause significant reduction in fruit yield and quality. With this study, the presence in Shaanxi was investigated of Apple stem pitting virus (ASPV), one of the economically important disease ...
Li, Z.N.   +4 more
openaire   +1 more source

APPLE STEM PITTING VIRUS DETECTION FROM DORMANT POME FRUITS BY RT-PCR

Acta Horticulturae, 2004
Apple stem pitting foveavirus is a major latent virus of pome fruits, and thus its reliable diagnostics is a crucial step in virus-free material production and prevention of ASPV spread by infected graftwoods. Thus it would be very convenient a relatively simple, accurate and rapid method in routine testing.
Paunović, Svetlana A.   +1 more
openaire   +2 more sources

IDENTIFICATION OF GENOME RECOMBINATION AMONG APPLE STEM PITTING VIRUS ISOLATES

Journal of Plant Pathology, 2016
We have determined the complete genome sequence of an apple stem pitting virus isolate (ASPV, genus Foveavirus) from China, providing a new genome sequence of an ASPV isolate from apple trees. Nucleotide identities and phylogenetic relationships among this and another eleven complete sequences of ASPV isolates largely differed according to the coding ...
Li, Z.   +5 more
openaire   +1 more source

Evaluation of several RT-PCR primer pairs for the detection of Apple stem pitting virus

Journal of Virological Methods, 2010
Detection of Apple stem pitting virus (ASPV) using RT-PCR based methods was studied in infected apple and pear trees. Three virus-specific primers (ASPF1CP, ASPF2CP, ASPR3CP) were designed to target the most conservative regions of the coat protein gene of 10 virus isolates in Poland and 7 other ASPV sequences available in GenBank.
B, Komorowska   +2 more
openaire   +2 more sources

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