Results 131 to 140 of about 493 (154)

Complete genomic sequence analyses of Apple stem pitting virus isolates from China

Virus Genes, 2011
The complete genomic sequences of a Chinese ASPV isolates KL1 and KL9 were determined from ten overlapping cDNA clones. The genomes of both isolates were 9265 nucleotides excluding the poly (A) tail and contained five open reading frames (ORFs). The identities between two complete genomes were 92.5% at nt level.
Na, Liu, Jianxin, Niu, Ying, Zhao
openaire   +2 more sources

Preparation of Antiserum to Apple stem pitting virus Using in Silico Prediction of Antigenic Epitopes

Journal of Phytopathology, 2011
Lili Li, Hongyi Yang
exaly   +2 more sources

BACK-TRANSMISSION OF A VIRUS ASSOCIATED WITH APPLE STEM PITTING AND PEAR VEIN YELLOWS, FROM NICOTIANA OCCIDENTALIS TO APPLE AND PEAR INDICATORS

Acta Horticulturae, 1995
The successful back-transmission of the mechanically transmissible virus associated with apple stem pitting and pear vein yellows, from Nicotiana occidentalis to apple seedlings "Golden Delicious" under greenhouse conditions is reported. This result enabled a field experiment where isolates of apple stem pitting, originating from apple, that were back ...
Leone, G., Lindner, J.L., Jongedijk, G., van der Meer, F.   +3 more
openaire   +2 more sources

Molecular characterization and variability analyses of apple stem pitting virus and apple stem grooving virus isolates infecting apple in Kashmir, India

Indian Phytopathology, 2022
Rashmi Suman, Narayan Rishi, Sunny Dhir, Vipin Hallan, Vanita Chandel   +4 more
openaire   +1 more source

A portable detection assay for Apple stem pitting virus using reverse transcription-recombinase polymerase amplification

Journal of Virological Methods, 2019
A molecular diagnostic assay for the rapid, sensitive and specific detection of Apple stem pitting virus (ASPV) in infected samples, utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 42 °C and the designed target-specific primers, was developed. The RT-RPA assay was able to be used in
Nam-Yeon, Kim, Hyo-Jeong, Lee, Rae-Dong, Jeong   +2 more
openaire   +2 more sources

Studies on cDNA Probe Detection Technology for Apple Stem Pitting Virus in Kuala Pear

Agricultural Sciences in China, 2007
Abstract Using Kuala pear leaves and cortexes as materials, the total RNA was extracted using two methods and these two methods were compared. The most suited methods for Kuala pear were screened; and biotin-labeled cDNA probe was synthesized using RT-PCR. The main factors that affected the sensitivity of hybridization were studied. Studies indicated
Jian-xin NIU, Jun ZHU, Wei-ming QIN, Bing-gang MA, Xiao-bing WANG, Zhong-hua WU   +5 more
exaly   +2 more sources

Apple stem pitting virus (apple Spy 227 epinasty & decline)

PlantwisePlus Knowledge Bank, 2022
openaire   +1 more source

Occurrence, distribution, and molecular characterization of apple stem pitting virus in Iran

2017
Apple Stem Pitting Virus (ASPV) is one of the most common viruses of apple and pear trees. During 2012 to 2013, a total of 1053 symptomatic and asymptomatic leaf samples were collected from orchards in nine provinces of Iran. ASPV infection was detected by DAS-ELISA and PCR in 54 samples (5.12%) from seven provinces.
Abtahi, F., Shams Bakhsh, M., Safaie, N., Autonell, C. R., RATTI, CLAUDIO   +4 more
openaire   +1 more source

Development and preliminary application of a reverse-transcription droplet digital PCR assay for detection and quantification of apple stem pitting virus in vitro propagated apple plantlets

Scientia Horticulturae, 2023
Rae-Dong Jeong
exaly  

Genetic diversity of latent Apple Stem Pitting Virus in Lithuania

2014
Mažeikienė, Ingrida, Jūrate Bronė Šikšnianienė   +1 more
openaire   +1 more source