Results 101 to 110 of about 19,186 (244)
DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis
Angewandte Chemie International Edition, EarlyView.Pyrimidine‐rich ssDNA flaps on the T7 promoter inhibit T7 RNA polymerase (T7RP) transcription. Leveraging this pyrimidine bias, we developed DNAzyme‐ and MNAzyme‐mediated flap promoter induced transcription control (D‐FIT and M‐FIT) systems. These platforms enable regulated transcription and sequence‐specific detection, highlighting the programmable ...
Eun Sung Lee +5 more
wiley +1 more source
Fluorescence Sensing Using DNA Aptamers in Cancer Research and Clinical Diagnostics [PDF]
, 2017 Among the various advantages of aptamers over antibodies, remarkable is their ability to tolerate a large number of chemical modifications within their backbone or at the termini without losing significant activity.
Moccia, Federica +4 more
core +2 more sources
Frontiers in Chemistry, 2015 Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target.
Emmanuelle eFiore +4 more
doaj +1 more source
A modular and extensible RNA-based gene-regulatory platform for engineering cellular function [PDF]
, 2007 Engineered biological systems hold promise in addressing pressing human needs in chemical processing, energy production, materials construction, and maintenance and enhancement of human health and the environment. However, significant advancements in our
Smolke, Christina D., Win, Maung Nyan
core +2 more sources
Developments in the tools and methodologies of synthetic biology. [PDF]
, 2014 Synthetic biology is principally concerned with the rational design and engineering of biologically based parts, devices, or systems. However, biological systems are generally complex and unpredictable, and are therefore, intrinsically difficult to ...
Freemont, P +3 more
core +1 more source
Selection Of A Novel Aptamer Against Vitronectin Using Capillary Electrophoresis And Next Generation Sequencing [PDF]
, 2016 Breast cancer (BC) results in ≃40,000 deaths each year in the United States and even among survivors treatment of the disease may have devastating consequences, including increased risk for heart disease and cognitive impairment resulting from the toxic ...
Boyacioglu, O. +8 more
core +3 more sources
Nanoclay‐Engineered Scaffolds for the Controlled Delivery of Biomolecules in Regenerative Medicine
Asia-Pacific Journal of Chemical Engineering, EarlyView.ABSTRACT Regenerative medicine combines biomaterials, cells, scaffolds, and bioactive agents via modern technologies to aid in the reconstruction and repair of damaged tissues. Among these, nanoclay scaffolds have demonstrated unique advantages in facilitating the delivery of therapeutic agents.
Mehri Shadi +2 more
wiley +1 more source
Surface plasmon resonance imaging for affinity-based biosensors [PDF]
, 2010 SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic ...
Mascini, Marco +3 more
core +1 more source
Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy
Biotechnology and Bioengineering, EarlyView.Schematic depicting the workflow for model building and validation using Raman spectroscopy for real‐time monitoring of IVT. ABSTRACT The success of COVID‐19 mRNA vaccines showcased the transformative potential of this adaptable, plug‐and‐play, and rapid response platform.
Julia Puppin Chaves Fulber +4 more
wiley +1 more source
Supramolecular Aptamers on Graphene Oxide for Efficient Inhibition of Thrombin Activity
Frontiers in Chemistry, 2019 Graphene oxide (GO), a two-dimensional material with a high aspect ratio and polar functional groups, can physically adsorb single-strand DNA through different types of interactions, such as hydrogen bonding and π-π stacking, making it an attractive ...
Ting-Xuan Lin +10 more
doaj +1 more source