Results 101 to 110 of about 581,942 (266)

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells

Advanced Science, EarlyView.
A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Mengru Lin, Jiale Sun, Yuqing Mao, Yuhao Tang, Fuan Wang, Zhihong Liu, Jing Wang   +6 more
wiley   +1 more source

Aptamers as theranostic agents: modifications, serum stability and functionalisation [PDF]

, 2013
Aptamers, and the selection process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX) used to generate them, were first described more than twenty years ago.
Adler, Bagalkot, Bagalkot, Bentolila, Bunka, Campbell, Cerchia, Chelliserrykattil, Cheng, ChunGuang Li, Crouzier, Damha, Davies, Dean, Djordjevic, Doessing, Doessing, Dongxi Xiang, Ellington, Gao, Gold, Hadi Al.Shamaileh, Hofacker, Hou, Imaizumi, Joanna Macdonald, Kang, Kasahara, Kasahara, Kasahara, Kato, Kaur, Keefe, Koshkin, Kusser, Kuwahara, Kuwahara, Lee, Lee, Levy, Liang Qiao, Lingxue Kong, Mallikaratchy, Mallikaratchy, McNamara, Mi, Michael O'Connor, Michalet, Ming Wei, Mohan, Obika, Padilla, Padilla, Pasternak, Paterson, Pendergrast, Petersen, Peyman, Pinheiro, Robertson, Saenger, Samani, Santanu Bhattacharya, Sarah Shigdar, Sato, Schmidt, Sefah, Shangguan, Shangguan, Shi, Shigdar, Shigdar, Shigdar, Shoji, Shu-Feng Zhou, Song, Sorensen, Stephenson, Tao Wang, Texier, Tuerk, Vaught, Veedu, Wang, Wei Duan, White, Wilson, Yimin Zhu, Zhang   +88 more
core   +3 more sources

Dynamic Decoration of DNA Scaffolds for High‐Resolution Cancer Cell Subtyping

Advanced Science, EarlyView.
This study presents a dual‐mode imaging platform that utilizes self‐assembly and disassembly of DNA scaffolds for high‐resolution cancer cell detection. The structural rigidity of DNA scaffolds effectively prevents cellular internalization for over 300 min.
Xiaolin Hu, Jie Xie, Xinlin Guo, Liangting Wang, Zhengheng Yu, Xiaopei Qiu, Heng Li, Kang Wang, Xiaoxing Wang, Mingxuan Song, Junsong Guo, Wei Gu, Sergio Bernardini, Chaoyong Yang, Hong Zhang, Yang Luo   +15 more
wiley   +1 more source

The prebiotic evolutionary advantage of transferring genetic information from RNA to DNA. [PDF]

, 2011
In the early 'RNA world' stage of life, RNA stored genetic information and catalyzed chemical reactions. However, the RNA world eventually gave rise to the DNA-RNA-protein world, and this transition included the 'genetic takeover' of information storage ...
Chen, Irene A, Gerland, Ulrich, Leu, Kevin, Obermayer, Benedikt, Rajamani, Sudha   +4 more
core   +4 more sources

Intelligent Thermo‐Self‐Limited Magnetothermia with Heat‐Triggered TERT Silencing for Precision Synergetic Cancer Therapy

Advanced Science, EarlyView.
Telomerase reverse transcriptase (TERT) mRNA induces DNA self‐assembly in cancer cells, boosting the nanoplatform's magnetothermal properties. This magnetothermia then activates the Hsp70 promoter, initiating siRNA synthesis to silence the TERT gene, enhancing cancer treatment.
Liang Zhang, Mingfu Gong, Tao Sun, Shilin Xiao, Yue Zhao, Xiaofeng Yang, Wansu Zhang, Chunyu Zhou, Xu Liu, Dong Zhang   +9 more
wiley   +1 more source

Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy [PDF]

, 2007
The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The
Haitang Li, Jiehua Zhou, John Rossi, John Zaia, Shirley Li   +4 more
core   +2 more sources

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Advanced Science, EarlyView.
Most rare diseases are caused by genetic mutations. Prime editing (PE) has emerged as a versatile tool capable of inducing diverse mutations without generating DNA double‐strand breaks. Despite its significant clinical potential, PE faces limitations in terms of efficiency and scalability.
Jihyeon Yu, Ju‐Chan Park, Heesoo Uhm, Yong‐Woo Kim, Hyeon Woo Im, Sangsu Bae   +5 more
wiley   +1 more source

Fluorescence Sensing Using DNA Aptamers in Cancer Research and Clinical Diagnostics [PDF]

, 2017
Among the various advantages of aptamers over antibodies, remarkable is their ability to tolerate a large number of chemical modifications within their backbone or at the termini without losing significant activity.
Moccia, Federica, Montesarchio, Daniela, Musumeci, Domenica, Platella, Chiara, Riccardi, Claudia   +4 more
core   +2 more sources

Identification of Amino Acids and Nucleotides Required for Interaction Between BAFF‐R and its RNA Aptamer

The FASEB Journal, 2015
Current cancer therapies such as surgery, radiation, and chemotherapy can leave residual tumor cells and have off‐target effects. Aptamers are used in a type of targeted cancer therapy that more precisely identify and attack cancer cells, while leaving unaffected cells unharmed. In B‐cell malignancies, such as non‐Hodgkins lymphoma,
Monica Lares, John Rossi
openaire   +1 more source

Dual Aptamers‐Based SETDB1 PROTACs as Effective Anti‐Tumor Strategies for Breast Cancer

Advanced Science, EarlyView.
This study establishes dual‐aptamer PROTACs targeting SETDB1 using a SETDB1‐specific aptamer conjugated to AS1411. The designed PROTACs penetrate cells, recruit MDM2 to degrade SETDB1, and inhibit cancer cell proliferation and migration. Remarkably, they also overcome tamoxifen resistance and enhance CD8+ T cell cytotoxicity, suppressing tumor growth ...
Yanxuan Guo, Yingge Lv, Shuyu Huang, Chang Liu, Yan Ouyang, Bei Lan, Chenghao Xuan   +6 more
wiley   +1 more source