Results 161 to 170 of about 15,557 (213)

Arachidonate 12-lipoxygenases with reference to their selective inhibitors

Biochemical and Biophysical Research Communications, 2005
Lipoxygenase is a dioxygenase recognizing a 1-cis,4-cis-pentadiene of polyunsaturated fatty acids. The enzyme oxygenates various carbon atoms of arachidonic acid as a substrate and produces 5-, 8-, 12- or 15-hydroperoxyeicosatetraenoic acid with a conjugated diene chromophore.
Shozo, Yamamoto, Michiko, Katsukawa, Ayumi, Nakano, Emi, Hiraki, Kohji, Nishimura, Mitsuo, Jisaka, Kazushige, Yokota, Natsuo, Ueda   +7 more
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Localization of arachidonate 12-lipoxygenase in canine brain tissues

Neuroscience Research Supplements, 1991
Abstract: The cytosol fraction from a thoroughly imgated canine cerebrum was subjected to immunoaffinity chromatography using a monoclonal antibody against porcine leukocyte 12‐lipoxygenase. Arachidonate 12‐lipoxygenase eluted from the column with some retardation. The enzyme, with a specific activity of 9 nmol/min/mg of protein, converted arachidonic
M, Nishiyama, H, Okamoto, T, Watanabe, T, Hori, T, Hada, N, Ueda, S, Yamamoto, H, Tsukamoto, K, Watanabe, T, Kirino   +9 more
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Mammalian Arachidonate 12-Lipoxygenases

1997
Since arachidonate 12-lipoxygenase was earlier found in platelets as the first mammalian lipoxygenase, the enzyme has been found in a variety of tissues of a number of animal species. On the basis of our studies in the last decade, we have demonstrated that there are two isoforms of 12-lipoxygenase (1).
S, Yamamoto, Y, Takahashi, T, Hada, H, Hagiya, H, Suzuki, G R, Reddy, N, Ueda, T, Arakawa, M, Nakamura, S, Matsuda, Y, Taketani, T, Yoshimoto, T, Azekawa, Y, Morita, K, Ishimura, S, Arase, W C, Glasgow, A R, Brash, M, Anton, H, Kühn   +19 more
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Arachidonate 12-Lipoxygenase Isozymes

1999
A lipoxygenase is an enzyme which incorporates one molecule of oxygen into arachidonic acid or other polyunsaturated fatty acids producing hydroperoxy acids with a conjugated diene system. Enzymes oxygenating at the positions 5, 8, 12 and 15 of arachidonic acid and producing the corresponding hydroperoxy eicosatetraenoic acid (HpETE) have been isolated
S, Yamamoto, H, Suzuki, M, Nakamura, K, Ishimura   +3 more
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Molecular cloning and expression of human arachidonate 12-lipoxygenase

Biochemical and Biophysical Research Communications, 1990
The cDNA for a 12-lipoxygenase was isolated from cDNA library of human erythroleukemia cells. The cDNA had an open reading frame encoding 663 amino acids with a calculated molecular weight of 75,513. The deduced amino acid sequence of human 12-lipoxygenase exhibited 41.5%, 65.3% and 65.4% identity with human 5-lipoxygenase, human 15-lipoxygenase and ...
T, Yoshimoto, Y, Yamamoto, T, Arakawa, H, Suzuki, S, Yamamoto, C, Yokoyama, T, Tanabe, H, Toh   +7 more
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Gossypol, a potent inhibitor of arachidonate 5- and 12-lipoxygenases

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1985
Gossypol inhibited 5- and 12-lipoxygenases of rat basophilic leukemia (RBL-1) cells with ID50 of 0.3 microM and 0.7 microM, respectively. Nearly two orders of magnitude of higher concentration of gossypol was required to inhibit prostaglandin synthetase. The inhibition was of a non-competitive type with respect to arachidonate.
Y, Hamasaki, H H, Tai
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Platelet-Type Arachidonate 12-Lipoxygenase

1996
In human platelets arachidonic acid is released from membrane phospholipids by the catalysis of phosopholipase A2, and then oxygenated either by cyclooxygenase or by 12-lipoxygenase. It is well known that the cyclooxygenase pathway leads to the synthesis of pro-aggregatory and vasoconstrictive thromboxane A2.
M, Nakamura, N, Ueda, S, Yamamoto, K, Ishimura, K, Tomo, M, Okuma   +5 more
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Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase

Blood, 2000
Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities on vascular endothelial cells ...
D, Nie, K, Tang, C, Diglio, K V, Honn
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Diurnal Change of Arachidonate 12-Lipoxygenase in Rat Pineal Gland

Biochemical and Biophysical Research Communications, 1997
Rat pineal gland contains a 12-lipoxygenase as demonstrated by the enzyme activity, RNA blot analysis and in situ hybridization. Using rats maintained with 12-h dark and light cycles, dynamic changes of the enzyme in pineal gland were examined. When the crude extract of pineal glands was incubated with arachidonic acid and the reaction products were ...
H, Kawajiri, N, Qiao, D M, Zhuang, T, Yoshimoto, H, Hagiya, S, Yamamoto, H, Sei, Y, Morita   +7 more
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Arachidonate 12-Lipoxygenase of Human Platelets

1993
Thromboxane A2 is well known as a proaggregatory and vasoconstrictive compound [1]. It is produced from arachidonic acid in platelets, and fatty acid cyclooxygenase responsible for the thromboxane synthesis is localized in the microsomal fraction of human platelets [2]. There is another metabolic pathway of arachidonic acid in human platelets.
Shozo Yamamoto, Satoshi Matsuda, Jun Murakami, Yasuchika Yamamoto, Yumiko Konishi, Chieko Yokoyama, Toshiya Arakawa, Yoshitaka Takahashi, Tanihiro Yoshimoto, Yasuo Mimura, Minoru Okuma   +10 more
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