Results 171 to 180 of about 15,557 (213)

Inhibition of platelet arachidonic acid 12-lipoxygenase by acetylenic acid compounds

Prostaglandins, 1981
Eighteen acetylenic fatty acids were tested as inhibitors of human platelet arachidonic acid 12-lipoxygenase. 4,7,10,13-Eicosatetraynoic (4,7,10,13-ETYA) acid emerged as the most potent compound. Additional experiments have shown that 4,7,10,13-ETYA selectively blocked the 12-lipoxygenase in washed human platelets with lesser activity against the ...
F F, Sun, J C, McGuire, D R, Morton, J E, Pike, H, Sprecher, W H, Kunau   +5 more
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Stimulation by carrageenan of arachidonate 12-lipoxygenase activity in dog gingival tissue

Archives of Oral Biology, 1991
At 4 h after injection of carrageenan into the gingiva, the 12-lipoxygenase activity of the gingival homogenate was markedly increased. Activity in the cytosol and microsomal fractions was markedly increased when assessed as the specific activity based on nmol/min/mg of protein, and in the cytosol fraction as the percentage distribution of total ...
K, Tamai, T, Dohi, H, Yoshino, M, Shirakawa, H, Okamoto, A, Tsujimoto   +5 more
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Lipoxin synthesis by arachidonate 12-lipoxygenase purified from porcine leukocytes

Biochemical and Biophysical Research Communications, 1987
Arachidonate 12-lipoxygenase purified from porcine leukocytes shows 14R-oxygenase and 14, 15-leukotriene A synthase activities with 15-hydroperoxy-arachidonic acid as substrate. The enzyme transformed 5, 15-dihydroperoxy-arachidonic acid to several compounds with a conjugated tetraene. A major product was identified as 5S, 14R, 15S-trihydroperoxy-6, 10,
N, Ueda, C, Yokoyama, S, Yamamoto, B J, Fitzsimmons, J, Rokach, J A, Oates, A R, Brash   +6 more
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Hinokitiol, a Selective Inhibitor of the Platelet-Type Isozyme of Arachidonate 12-Lipoxygenase

Biochemical and Biophysical Research Communications, 2000
Hinokitiol (4-isopropyltropolone), a constituent of Japanese cypress, reversibly inhibited platelet-type 12-lipoxygenase with an IC(50) of 0.1 microM, and the enzyme activity was almost lost at 1 microM. The compound was much less active with other lipoxygenase enzymes with higher IC(50) values (leukocyte-type 12-lipoxygenase, 50 microM; soybean ...
H, Suzuki, T, Ueda, I, Juránek, S, Yamamoto, T, Katoh, M, Node, T, Suzuki   +6 more
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Selective expression of an arachidonate 12-lipoxygenase by pancreatic islet beta-cells

American Journal of Physiology-Endocrinology and Metabolism, 1992
The immunohistochemical distribution of arachidonate lipoxygenases in rat pancreas was characterized with specific polyclonal anti-5-lipoxygenase and anti-12-lipoxygenase antibodies. Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat pancreas using anti-12-lipoxygenase antibody and biotin-avidin-peroxidase detection demonstrated ...
V R, Shannon, S, Ramanadham, J, Turk, M J, Holtzman   +3 more
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Arachidonate 12-Lipoxygenases: Enzymology and Molecular Biology

1993
In contrast to 5-lipoxygenase which is involved in the biosynthesis of bioactive leukotrienes, a general physiological role of 12-lipoxygenase has not yet been established. Although the enzyme was earlier found in platelets as the first mammalian lipoxygenase and has been found in various tissues of many animal species, most investigators have ignored ...
S. Yamamoto, T. Yoshimoto, N. Ueda, Y. Takahashi, S. Matsuda, H. Suzuki, T. Oshima, H. Ikawa, T. Hada, G. R. Reddy, Y. Yamamoto, K. Kishimoto, T. Arakawa, K. Ishimura, S. Arase, C. Yokoyama, T. Tanabe, H. Toh, M. Nishiyama, H. Okamoto, T. Watanabe, T. Hori   +21 more
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Arachidonate 12-lipoxygenase of porcine leukocyte with activity for 5-hydroxyeicosatetraenoic acid

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
When arachidonic acid was incubated with porcine polymorphonuclear leukocytes, 12-hydroxy-5,8,10,14-eicosatetraenoic acid was produced as a major product. The production of this compound was not accounted for by contaminating platelets known to contain arachidonate 12-lipoxygenase. The enzyme was found predominantly in a cytosol fraction of leukocytes,
T, Yoshimoto, Y, Miyamoto, K, Ochi, S, Yamamoto   +3 more
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Analysis of non-heme iron in arachidonate 12-lipoxygenase of porcine leukocytes

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
Arachidonate 12-lipoxygenase of porcine leukocytes, which was purified to homogeneity by immunoaffinity chromatography, was analyzed for iron content by atomic absorption spectrophotometry. The enzyme contained 0.70 +/- 0.09 g atom of iron per mol of enzyme (mean +/- S.D., n = 4).
S, Matsuda, H, Suzuki, T, Yoshimoto, S, Yamamoto, A, Miyatake   +4 more
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Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
Incubation of rat lung cytosol with arachidonic acid produced 12-hydroxy-5,8,10,14-eicosatetraenoic acid as a major product, which was identified by gas chromatography-mass spectrometry. By ammonium sulfate fractionation and DEAE-cellulose chromatography the arachidonate 12-lipoxygenase was purified about 30-fold from the rat lung cytosol.
C, Yokoyama, K, Mizuno, H, Mitachi, T, Yoshimoto, S, Yamamoto, C R, Pace-Asciak   +5 more
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Docking arachidonic acid into human 5/12-lipoxygenase using ICM

2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops (BIBMW), 2011
Lipoxygenases(LOX) are a super gene family of iron-containing enzymes, that catalyze the stereo- and regio-specific formation of fatty acid hydroperoxides from polyunsaturated fatty acids. Mammalian lipoxygenase have been implicated in the pathogenesis of various inflammatory responses.
Mingmin Bai, Ebrahim Khosravi, Shuju Bai
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