Results 161 to 170 of about 113,343 (263)

Structural and mutational analysis of Methanosarcina mazei prenylated FMN synthase reveals the basis of its unique prenyl donor substrate specificity

The FEBS Journal, EarlyView.
Some prenylated flavin mononucleotide (prFMN) synthases exceptionally prefer dimethylallyl phosphate (DMAP) over dimethylallyl diphosphate (DMAPP), which is a common prenyl donor substrate for many prenyltransferases. Structural and mutagenic analyses of Methanosarcina mazei prFMN synthase, which prefers DMAP but can also accept DMAPP, elucidated its ...
Sou Fukuhara, Hideaki Unno, Soma Ishimine, Ryuhei Nagata, Tomokazu Ito, Hisashi Hemmi   +5 more
wiley   +1 more source

Physiology and ecology of Methanogenic Archaea

, 2014
The aim of this study was to obtain the available information about physiology and ecology of methanogenic Archaea. Determine quantity of methanogens in relation to different types of peatlands and different depths of soil.
LÍSALOVÁ, Olga
core  

Rescue of pATOM36‐depleted T. brucei by human MTCH1/2 reveals common features of protein insertases

The FEBS Journal, EarlyView.
Human MTCH1 and MTCH2 (with an N‐terminal extension) functionally complement the loss of pATOM36 in T. brucei, indicating that these evolutionarily distant proteins recognize a common set of substrates and share fundamental principles of mitochondrial outer membrane protein insertion. Structural analyses reveal a common hydrophilic cavity, supporting a
Stephan Berger, Markus Daniel Gerber, Torsten Ochsenreiter, Christoph von Ballmoos, André Schneider   +4 more
wiley   +1 more source

Domain classification of archaeal proteomes reveals conserved fold repertoire. [PDF]

PLoS Comput Biol
Schaeffer RD, Pei J, Guo R, Zhang J, Medvedev K, Cong Q, Grishin N.   +6 more
europepmc   +1 more source

Crystal structure of human NIF3‐like protein reveals dynamic hexameric assembly with a single divalent metal binding site

The FEBS Journal, EarlyView.
We present the first crystal structures of human NIF3, revealing a dynamic, hexameric ring with a central cavity regulated by flexible insertion domains. Crucially, human NIF3 features a mononuclear zinc‐binding site, marking a distinct evolutionary departure from the dinuclear centers of bacterial homologs.
Elżbieta Wątor‐Wilk, Krzysztof Żak, Piotr Wilk, Paweł Kochanowski, Agnieszka Maślanka, Łukasz Skalniak, Przemysław Grudnik   +6 more
wiley   +1 more source

Large-scale benchmarking of prokaryotic annotation tools across thousands of species. [PDF]

Genome Biol
Jundzill M, Hölzer M, Mangul S, Marquet M, Ehricht R, Lohde M, Spott R, Makarewicz O, Pletz MW, Brandt C.   +9 more
europepmc   +1 more source

Crystal structure and biochemical analysis of the polyester‐degrading carboxylesterase SM0281 from the symbiotic legume‐associated rhizobacterium Sinorhizobium (Ensifer) meliloti

The FEBS Journal, EarlyView.
The microbial degradation of synthetic polyesters such as polyethylene terephthalate (PET) is mediated by diverse α/β‐hydrolases, many of which remain poorly characterized. In this study, we investigated the α/β‐hydrolase SM0281 from Sinorhizobium meliloti and found that it exhibits robust carboxylesterase and polyesterase activities toward PET, PCL ...
Sofia Lemak, Anna N. Khusnutdinova, Peter J. Stogios, Elena Evdokimova, Alexei Savchenko, Peter N. Golyshin, Elizabeth A. Edwards, Alexander F. Yakunin   +7 more
wiley   +1 more source

Seasonal Variations in Microbial Community Structure and Function in the Waters Along the Yangtze-to-Huaihe Water Diversion Project According to Metagenomics. [PDF]

Microorganisms
Chen H, Xu B, Xie Q, Gu Z, Guo S, Chen S.   +5 more
europepmc   +1 more source

Hydrothermal origin of metabolic phosphorylation

The FEBS Journal, EarlyView.
Serpentinizing hydrothermal vents are candidate sites for biochemical origins. They generate extremely reducing conditions, depositing phosphite (HPO32−) and transition metals, including palladium, in the native (zero‐valent) state. Schlikker et al.
Manon L. Schlikker, Nadja K. Hoffmann, Sabine Metzger, Jorge Moral‐Pombo, Harun Tüysüz, William F. Martin   +5 more
wiley   +1 more source

Catalysis and allostery in bacterial ATP phosphoribosyltransferases

The FEBS Journal, EarlyView.
ATP phosphoribosyltransferase (ATPPRT) catalyses the first reaction of L‐histidine biosynthesis. L‐Histidine allosterically inhibits ATPPRT to regulate this energy‐intensive pathway in histidine‐synthesising organisms, such as bacteria, archaea and plants.
Lillian G. Bates, Rafael G. da Silva
wiley   +1 more source