Results 161 to 170 of about 103,243 (303)

What’s in our bin?

EMBO Reports
An analysis by Green Labs Austria shows the amount and content of plastic waste generated by life science research. To reduce waste and help to address global plastic pollution, labs should proactively explore alternatives and recycling methods.
Philipp M Weber, Cleophea Michelsen, Melina Kerou   +2 more
doaj   +1 more source

S‐Adenosylmethionine (SAM) hydrolases counter increased SAM epimerisation in thermophilic archaea

The FEBS Journal, EarlyView.
S‐Adenosyl‐l‐methionine (SAM) is a vital enzyme cofactor. Epimerisation at the sulfonium centre of biologically active (SS,SCα)‐SAM is driven by heat, yielding biologically inactive (RS,SCα)‐SAM. Here, two novel archaeal SAM hydrolases from the thermophilic Sulfolobus acidocaldarius and the halophilic Haloferax volcanii are shown to cleave (RS,SCα)‐SAM.
Agnes Bartels, Michael K. F. Mohr, Phillip Nußbaum, Marie Joest, Bianca Wassmer, Laurent Rasquin, Sonja‐Verena Albers, Jennifer N. Andexer   +7 more
wiley   +1 more source

Cryo‐EM structures of multiple‐peptide resistance factor (MprF) from Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
Cryo‐EM analysis of MprF from Pseudomonas aeruginosa reveals a dimeric enzyme, distinct from Rhizobium homologues. The soluble GNAT domain contains a dynamic amphipathic helix (TTH) that adopts different positions in detergent micelle and nanodisc, relative to the membrane.
Shaileshanand Jha, Kutti R. Vinothkumar
wiley   +1 more source

Hsp70 diversification and repurposing across the tree of life: Lessons from the evolutionary and mechanistic trajectory of the Hsp70–Hsp110 chaperone system

The FEBS Journal, EarlyView.
Evolutionary and mechanistic divergence in the Hsp70–Hsp110 chaperone system. Prokaryotic Hsp70s probably diversified into multiple orthologues that cooperated with co‐chaperones such as JDPs and NEF, forming increasingly complex proteostasis networks.
Pierre Goloubinoff, Paolo De Los Rios, Mathieu E. Rebeaud   +2 more
wiley   +1 more source

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source

Prebiotic aqueous reactions catalyzed by native nickel without hydrogen

The FEBS Journal, EarlyView.
Serpentinizing (H2‐producing) hydrothermal vents are candidate environments for metabolic origin. They generate highly reducing conditions that convert CO2 to formate and methane in abiotic reactions resembling reactions of the acetyl‐CoA pathway of CO2 fixation. They also contain natural catalysts. Native nickel (Ni0), like Fe0, Co0, and their alloys,
Carolina Garcia Garcia, Max Brabender, William F. Martin   +2 more
wiley   +1 more source

Diet breadth shapes gut microbiota in the invasive hornet Vespa velutina

Insect Conservation and Diversity, EarlyView.
Dietary and microbial profiles of V. velutina larvae are dominated by Apidae and Firmicutes, respectively. DNA metabarcoding of larval meconium and gut samples reveals a significant positive correlation between prey richness and bacterial diversity in the invasive hornet V. velutina. Multiple significant correlations exist between dietary and microbial
Cayetano Herrera, Iris Alemany, Mar Leza, José A. Jurado‐Rivera   +3 more
wiley   +1 more source

Metagenomics and metatranscriptomics of prokaryotic and fungal microbiomes in produced water associated with petroleum degradation and pipeline corrosion from an oil terminal in Brazil. [PDF]

World J Microbiol Biotechnol
Gomes RF, García GJY, Cardoso MS, Dutra JDCF, de Abreu Waldow V, Akamine RN, de Sousa MP, Groposo C, Brenig B, Figueiredo H, de Carvalho Azevedo VA, Góes-Neto A.   +11 more
europepmc   +1 more source

Distinct Microbiota and Functional Pathway Profiles Define Success and Failure in Regenerative Endodontic Treatment

International Endodontic Journal, EarlyView.
ABSTRACT Aim To characterize the intracanal microbiota and identify key microbial taxa and functional pathways associated with regenerative endodontic treatment (RET) outcomes. Methodology In this observational cohort study, 196 samples were collected at five time points from external tooth surfaces (T1, T4) and root canals (T2, T3, T5).
Leimarembi Devi Naorem, Alina Wikström, Peter Quach, Lokeshwaran Manoharan, Ronald Ordinola‐Zapata, Olena Rakhimova, Malin Brundin, Nelly Romani Vestman   +7 more
wiley   +1 more source

Molecular Evolution of the Archaeal DNA-Dependent RNA Polymerase: Cooperative Changes in Subunit Composition and Specific Domains of Small Subunits. [PDF]

Int J Mol Sci
Shematorova EK, Shpakovski GV.
europepmc   +1 more source