Results 61 to 70 of about 112,166 (340)

TriCON: A Carbon‐Based Triple‐Modal Nanoplatform for Pancreatic Cancer Therapy

Advanced Science, EarlyView.
We developed TriCON, a triple‐modality nanotherapeutic platform, to treat pancreatic ductal adenocarcinoma (PDAC) by synergizing gene editing, chemotherapy, and immunotherapy. TriCON utilizes CRISPR/Cas9 to target the poliovirus receptor (PVR), combined with nano‐encapsulated doxorubicin and checkpoint blockade. This approach achieved significant tumor
Xinyu Peng, Jiaxing Huang, Shengnan Lv, Jian Zhang, Liangliang Zhang, Huan Liu, Yan Liu, Zhen‐An Qiao, Feng Wei, Bao‐Lian Su   +9 more
wiley   +1 more source

Wide diversity of methane and short-chain alkane metabolisms in uncultured archaea

Nature Microbiology, 2019
Methanogenesis is an ancient metabolism of key ecological relevance, with direct impact on the evolution of Earth’s climate. Recent results suggest that the diversity of methane metabolisms and their derivations have probably been vastly underestimated ...
Guillaume Borrel, Panagiotis S. Adam, L. McKay, Lin-Xing Chen, I. N. Sierra-Garcia, Christian M. K. Sieber, Quentin Letourneur, A. Ghozlane, G. Andersen, Wenjun Li, S. Hallam, G. Muyzer, V. M. de Oliveira, W. Inskeep, J. Banfield, S. Gribaldo   +15 more
semanticscholar   +1 more source

A Dynamic Gate Enables Regioselective Hydroxylation of Free Arginine by a Non‐Canonical Heme Enzyme

Advanced Science, EarlyView.
The YqcI/YcgG enzyme AglA employs a unique “sandwich” fold and a dynamic gating mechanism to selectively hydroxylate AMPn‐Arg at Nε. Site‐directed mutations reprogram regioselectivity or enable activity on free arginine by altering hydrogen‐bond networks, precisely positioning substrates.
Yuan Sun, Chao Dou, Weizhu Yan, Pengpeng Chen, Lu Zhang, Dan Zhou, Yanhui Zheng, Zhaolin Long, Shoujie Li, Xiaoqing Xu, Qiuxia Huang, Xiaofeng Zhu, Wei Cheng   +12 more
wiley   +1 more source

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing

PLoS ONE, 2014
For the analysis of microbial community structure based on 16S rDNA sequence diversity, sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate microbial composition data, PCR amplification must be free of bias; however,
Shunsuke Takahashi, Junko Tomita, K. Nishioka, Takayoshi Hisada, M. Nishijima   +4 more
semanticscholar   +1 more source

Principles for Rigorous Design and Application of Synthetic Microbial Communities

Advanced Science, EarlyView.
SynComs are artificially designed to enable inter‐species metabolic interactions, metabolic division of labor, and ecological interactions that can elicit phenotypes like colonization stability and environmental adaptation. This systematic review explores the processes used to construct SynComs, the assessment of the mechanisms of metabolic interaction
Yuxiao Zhang, Minyu Jing, Lihui Lyu, Li Nie, Xihui Xu, Ru Sun, Xiyuan Xu, Siyu Chen, Shuobing He, Yumeng Zhang, Ping Huang, Weijie Luo, Jiaojiao Liang, Guifeng Gao, Kunkun Fan, Teng Yang, Liyan Zhang, Xiao Fu, Sarah M. Allard, Jack A. Gilbert, Jiabao Zhang, Haiyan Chu   +21 more
wiley   +1 more source

Next Generation DNA-Seq and Differential RNA-Seq Allow Re-annotation of the Pyrococcus furiosus DSM 3638 Genome and Provide Insights Into Archaeal Antisense Transcription

Frontiers in Microbiology, 2019
Pyrococcus furiosus DSM 3638 is a model organism for hyperthermophilic archaea with an optimal growth temperature near 100°C. The genome was sequenced about 18 years ago.
Felix Grünberger, Robert Reichelt, Boyke Bunk, Cathrin Spröer, Jörg Overmann, Jörg Overmann, Reinhard Rachel, Dina Grohmann, Winfried Hausner   +8 more
doaj   +1 more source

Uncovering the temporal dynamics and regulatory networks of thermal stress response in a hyperthermophile using transcriptomics and proteomics

mBio, 2023
Facing rapid fluctuations in their natural environment, extremophiles, like the hyperthermophilic archaeon Pyrococcus furiosus, exhibit remarkable adaptability to extreme conditions.
Felix Grünberger, Georg Schmid, Zubeir El Ahmad, Martin Fenk, Katharina Vogl, Robert Reichelt, Winfried Hausner, Henning Urlaub, Christof Lenz, Dina Grohmann   +9 more
doaj   +1 more source

Reverse Methanogenesis and Respiration in Methanotrophic Archaea

Archaea, 2017
Anaerobic oxidation of methane (AOM) is catalyzed by anaerobic methane-oxidizing archaea (ANME) via a reverse and modified methanogenesis pathway. Methanogens can also reverse the methanogenesis pathway to oxidize methane, but only during net methane ...
P. Timmers, C. Welte, J. Koehorst, C. Plugge, M. Jetten, A. Stams   +5 more
semanticscholar   +1 more source

Mechanisms of Direct Electron Transfer Governed by Redox‐Active Conductive Carrier with Superior Wettability in Anaerobic Biofilms

Advanced Science, EarlyView.
A redox‐active conductive carrier of tannic acid‐modified iron‐biochar with superior wettability is demonstrated to regulate direct electron transfer at the anaerobic biofilm‐carrier interface by enhancing the cytochrome c‐mediated pathway. For the first time, integrated evidence from spectroscopy, electrochemical analyses, and metatranscriptomics ...
Junli Tian, Xiaoyuan Zhang, Lulu Xing, Bin Ji, Jinfeng Lu, Yu Liu   +5 more
wiley   +1 more source

Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under N2-fixing conditions

Scientific Reports
Methanogenic archaea (methanogens) possess fascinating metabolic characteristics, such as the ability to fix molecular nitrogen (N2). Methanogens are of biotechnological importance due to the ability to produce methane (CH4) from molecular hydrogen (H2 ...
Barbara Reischl, Benjamin Schupp, Hayk Palabikyan, Barbara Steger-Mähnert, Christian Fink, Simon K.-M. R. Rittmann   +5 more
doaj   +1 more source