Results 71 to 80 of about 103,243 (303)

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

open access: yesNature Communications, 2019
The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink   +5 more
doaj   +1 more source

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

open access: yesAdvanced Science, EarlyView.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan   +7 more
wiley   +1 more source

Molecular biology of hyperthermophilic Archaea [PDF]

open access: yes, 1998
The sequences of a number of archaeal genomes have recently been completed, and many more are expected shortly. Consequently, the research of Archaea in general and hyperthermophiles in particular has entered a new phase, with many exciting discoveries ...
MORACCI, Marco   +11 more
core  

Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon Methanosarcina mazei go1 [PDF]

open access: yes, 2008
Methanogenic archaea accumulate glycine betaine in response to hypersalinity, but the regulation of proteins involved, their mechanism of activation and regulation of the corresponding genes are largely unknown.
Schmidt, S.   +5 more
core   +1 more source

Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales

open access: yesBiomolecules, 2020
Prokaryotes are constantly coping with attacks by viruses in their natural environments and therefore have evolved an impressive array of defense systems.
Isabelle Anna Zink   +2 more
doaj   +1 more source

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

open access: yesAdvanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang   +8 more
wiley   +1 more source

The first head-tailed virus, MFTV1, produced by a hyperthermophilic methanogenic deep-sea archaea [PDF]

open access: yes, 2020
International audienceDeep-sea hydrothermal vents are inhabited by complex communities of microbes and their viruses. Despite the importance of viruses in controlling the diversity, adaptation and evolution of their microbial hosts, to date, only eight ...
Dupont, Samuel   +19 more
core   +1 more source

Metagenomic Association Uncovers Host Genotype‐Structured Rhizobacterial Networks and Novel Taxa That Enhance Soybean Salt Tolerance

open access: yesAdvanced Science, EarlyView.
An STI‐guided metagenomic association framework links soybean salt‐tolerance phenotypes with rhizosphere microbiome composition and network organization in natural saline soils. The approach identifies candidate beneficial taxa, including Thalassospira xiamenensis, and provides an efficient strategy for mining stress‐protective microbes from native ...
Yu Luo   +3 more
wiley   +1 more source

The archaea (2017) [PDF]

open access: yes, 2017
The Archaea’ features a series of constructed photographic tableaux of tangled, botanical phantasmagoria, which refer to the landscape with a suggestion of figuration.
Rushton, S.
core  

Influences of soil properties on archaeal diversity and distribution in the McMurdo Dry Valleys, Antarctica [PDF]

open access: yes, 2011
The Antarctic Dry Valleys are characterized by extremely low temperatures, arid conditions, high salinity and virtual absence of plants. Therefore, food webs of these microbially dominated soils are among the simplest on earth making these mineral soils ...
Richter, Ingrid
core  

Home - About - Disclaimer - Privacy