Results 81 to 90 of about 1,071,418 (268)
Architecture and Engineering, 2018 Biomimicry is an old approach, but in the scientific conceptualization is new, as an approach of innovation based on the emulation of Nature, in recent years, this approach brings many potential theories and innovations in the architecture field. Indeed,
Yassine Mohammed Benyoucef, Andrey Razin
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Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Bajo el signo de la esvástica. La Exposición de Arquitectura Moderna alemana en España (1942)
Diacronie. Studi di Storia Contemporanea, 2014 In autumn of 1942, Barcelona received the Exhibition of Modern German Architecture, directed by Albert Speer. The objective of this exhibition was to show to European allies the pharaonic projects of Germany, the Europa’s future new capital, the new ...
Francesc Vilanova i Vila-Abadal es Profesor Titular de Historia Contemporánea en la Universidad Autónoma de Barcelona y Director del Archivo Carles Pi i Sunyer (Fundació Carles Pi i Sunyer d’Estudis Autonòmics i Locals, Barcelon). Miembro del Grup de Recerca en l’Època Franquista (GREF) y del Centre d’Estudis sobre les Èpoques Franquista i Democràtica (UAB-CEFID).
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The problems of high-rise construction in St. Petersburg
Journal of Architecture and Urbanism, 2016 St. Petersburg’s strict limit on building height existed until the 1960s. A small loosening of restrictions occurred only at the end of the 19th century, which formed the “horizontal” silhouette of the city with a few spire accents, domes and factory ...
Leonid Lavrov, Fedor Perov
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Cultural landscape in theory. 1st Part: Centre, core and triangle [PDF]
Urbani Izziv, 2002 The article presents ideas about the close links between architectural forms in theory and practice, as well as the extraordinary ties between the aspects of various disciplines and architectural expression.
Borut Juvanec
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Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source