Results 41 to 50 of about 9,646 (208)
Role of the Arf6 GDP/GTP Cycle and Arf6 GTPase-activating Proteins in Actin Remodeling and Intracellular Transport [PDF]
Journal of Biological Chemistry, 2006 We have analyzed both biochemically and functionally a series of Arf6 mutants, providing new insights into the molecular mode of action of the small G protein Arf6. First, by comparing a fast-cycling mutant (Arf6(T157N)) and a GTPase-deficient mutant (Arf6(Q67L)), we established the necessity for completion of the Arf6 GDP/GTP cycle for recycling of ...
Stéphanie, Klein +3 more
openaire +2 more sources
Arf6-driven endocytic recycling of CD147 determines HCC malignant phenotypes
Journal of Experimental & Clinical Cancer Research, 2019 Background Adhesion molecules distributed on the cell-surface depends upon their dynamic trafficking that plays an important role during cancer progression. ADP-ribosylation factor 6 (Arf6) is a master regulator of membrane trafficking.
Shanshan Qi +9 more
doaj +1 more source
The structural GDP/GTP cycle of human Arf6 [PDF]
The EMBO Reports, 2001 The small GTP-binding protein Arf6 coordinates membrane traffic at the plasma membrane with aspects of cytoskeleton organization. This function does not overlap with that of other members of the ADP-ribosylation factor (Arf) family, although their switch regions, which are their major sites of interaction with regulators and effectors, have virtually ...
S, Pasqualato +3 more
openaire +2 more sources
, 2013 (A) ARF6 was bound to IL-15Rα. Cells were transfected with ARF6-HA 24 h earlier. ARF6 protein was immunoprecipitated and subjected to WB for IL-15 and IL-15Rα. Immunoprecipitation with nonspecific IgGs was done as the control.
Weihong Pan (327613) +3 more
core +1 more source
ARF6 regulates Nox1 expression.
, 2016 (A) Nox1 and Nox4 protein expression was examined in control and ARF6 depleted VSMC using Western blot analysis. Levels of ARF6 and actin were also determined. Graph represents quantification of all data (n = 3, ***P157A, HA-ARF6 T27N, myc-Rac1, myc-Rac1
Mohamed Bourmoum (2176606) +2 more
core +1 more source
PLoS ONE, 2012 BackgroundARF6 (ADP-ribosylation factor 6) small GTP binding protein plays critical roles in actin cytoskeleton rearrangements and membrane trafficking, including internalisation of G protein coupled receptors (GPCR). ARF6 operates by cycling between GDP-
Venkateswarlu Kanamarlapudi +3 more
doaj +1 more source
rNadA intracellular distribution is affected by ARF6.
, 2014 Chang cells were transfected in order to overexpress wild type ARF6 (ARF6-wt) or dominant-negative ARF6 (ARF6-Q67L) and then incubated overnight with 200 µg/ml rNadA at 37°C. Afterwards, cells were fixed, permeabilized and double stained for rNadA (green)
Rino Rappuoli (42469) +15 more
core +1 more source
Molecular modeling of ARF6 dysregulation caused by mutations in IQSEC2
, 2023 IQSEC2 gene mutations are associated with epilepsy, autism, and intellectual disability. The primary function IQSEC2, mediated via its Sec 7 domain, is to act as a guanine nucleotide exchange factor for ARF6. We sought to develop a molecular model, which
Andrew P. Levy (15331437) +7 more
core +1 more source
European Journal of Cell Biology, 2007 Actin cytoskeleton dynamics and membrane trafficking are tightly connected and are among the most important driving forces of neuronal development, basic synaptic transmission events, and synaptic plasticity. One group of proteins involved in coordination of these two processes is the family of ADP ribosylation factors (ARFs) regulating actin dynamics,
openaire +2 more sources
ARF6 controls RHOB targeting to endosomes regulating cancer cell invasion
Molecular & Cellular Oncology, 2020 Endocytic trafficking has emerged as an essential mechanism to spatiotemporally coordinate signaling protein complexes that control cytoskeletal dynamics and cell motility. Our study established an unexpected regulatory mechanism whereby ADP ribosylation
Kossay Zaoui +3 more
doaj +1 more source