Results 1 to 10 of about 192,846 (155)

Arginine and lysine as products of basic carboxypeptidase activity associated with fibrinolysis [PDF]

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2012
Blood carboxypeptidases play an important role in the regulation of fibrinolysis. We have proposed here the method for the assay of blood carboxypeptidase activity associated with coagulation/fibrinolysis using the natural substrate fibrin and the detection of basic amino acids arginine and lysine as products in the conditions close to those in vivo ...
Alexander Zhloba
exaly   +4 more sources

Anabaenopeptin B Chlorination Degradation By-Products Retain Potent Carboxypeptidase B Inhibition. [PDF]

ACS ES T Water
The release of anabaenopeptins (APs) into source freshwater from cyanobacteria harmful algal blooms (cHABs) has raised concerns due to their reported abundance and inhibitory activity toward carboxypeptidases (CP).
Stringer BB, Quandt ML, Thenuwara SI, Peraino NJ, Kodanko JJ, Westrick JA.   +5 more
europepmc   +2 more sources

Purification and Characterization of Arginine Carboxypeptidase Produced by Porphyromonas gingivalis [PDF]

Infection and Immunity, 2002
ABSTRACT Arginine carboxypeptidase was isolated from the cytoplasm of Porphyromonas gingivalis 381 and purified by DEAE-Sephacel column chromatography, followed by high-performance liquid chromatography on DEAE-5PW and TSK G2000SW XL .
K. Masuda, M. Yoshioka, D. Hinode, R. Nakamura   +3 more
semanticscholar   +3 more sources

Guanidine derivatives restore activity to carboxypeptidase lacking arginine‐127 [PDF]

Protein Science, 1992
AbstractArg‐127 stabilizes the oxyanion of the tetrahedral intermediate formed during Zn2+ carboxypeptidase A‐catalyzed hydrolysis. Mutant carboxypeptidases lacking Arg‐127 exhibit substantially reduced rates of hydrolysis with the change manifest almost entirely in kcat (kcat/Km is decreased by 104 for R127A).
M. Phillips, L. Hedstrom, W. Rutter
semanticscholar   +3 more sources

D-Arg0-Bradykinin-Arg-Arg, a Latent Vasoactive Bradykinin B2 Receptor Agonist Metabolically Activated by Carboxypeptidases

Frontiers in Pharmacology, 2018
We previously reported hypotensive and vasodilator effects from C-terminally extended bradykinin (BK) sequences that behave as B2 receptor (B2R) agonists activated by vascular or plasma peptidases.
Hélène Bachelard, Xavier Charest-Morin, François Marceau   +2 more
exaly   +3 more sources

The nature of the ligand's side chain interacting with the S1'-subsite of metallocarboxypeptidase T (from Thermoactinomyces vulgaris) determines the geometry of the tetrahedral transition complex.

PLoS ONE, 2019
The carboxypeptidase T (CPT) from Thermoactinomyces vulgaris has an active site structure and 3D organization similar to pancreatic carboxypeptidases A and B (CPA and CPB), but differs in broader substrate specificity.
Valery Kh Akparov, Vladimir I Timofeev, Galina E Konstantinova, Ilyas G Khaliullin, Inna P Kuranova, Tatiana V Rakitina, Vytas Švedas   +6 more
doaj   +2 more sources

Arginine Carboxypeptidase (CPR) in Human Plasma Determined with Sandwich ELISA

Microbiology and Immunology, 1999
AbstractThere are two types of carboxypeptidases present in human blood, carboxypeptidase N (CPN) and arginine carboxypeptidase (CPR). CPR is generated during coagulation from a precursor (proCPR) which can be converted to the active form by trypsin in vitro.
Xiao-yan Guo, A. Morioka, Y. Kaneko, N. Okada, K. Obata, Tomoyuki Nomura, W. Campbell, H. Okada   +7 more
semanticscholar   +3 more sources

Effect of Anticoagulants in Colorimetric Assay for Basic Carboxypeptidases

Microbiology and Immunology, 2002
H. Komura, K. Obata, W. Campbell, M. Yumoto, Y. Shimomura, H. Katsuya, N. Okada, H. Okada   +7 more
exaly   +2 more sources

Measurement of Arginine Carboxypeptidase‐Generating Activity of Adult Plasma [PDF]

Microbiology and Immunology, 1998
AbstractArginine carboxypeptidase (CPR) is a novel carboxypeptidase which was first described by Campbell and Okada. CPR is generated from a stable precursor of CPR (proCPR) during coagulation or under other circumstances and is promptly inactivated at 37 C. Therefore, it is not easy to determine CPR in blood samples. Since proCPR can be separated from
M, Watanabe, Y, Ishikawa, W, Campbell, H, Okada   +3 more
openaire   +3 more sources

Prodrugs Activated by Vascular Ectopeptidases: Proof of Concept

Medical Sciences Forum, 2022
Several vascular ectopeptidases reside in blood vessels and efficiently regulate peptide hormones. For instance, bradykinin (BK) is inactivated by angiotensin converting enzyme (ACE) or arginine-carboxypeptidases (Arg-CPs), and aminopeptidase N (APN ...
François Marceau
doaj   +1 more source