Results 71 to 80 of about 4,966,594 (348)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Fisheries‐induced evolution of alternative male life history tactics in Coho salmon

Evolutionary Applications, 2020
Fisheries‐induced evolution (FIE) can result when harvest imposes artificial selection on variation in heritable phenotypic traits. While there is evidence for FIE, it remains difficult to disentangle the contributions of within‐generation demographic ...
Kyle A. Young, Victoria A. Cluney, Laura K. Weir   +2 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Exploration of gene presence/absence variations in Oncorhynchus mykiss and their differentiation between wild and selection populations

Open Biology
Gene presence/absence variations (PAVs) have been considered as the important determinants of genome evolution and phenotypic diversity. However, studies on gene PAVs have been poorly documented, especially in fishes. In the present study, the pan-genome
Hancheng Bao, Na Xue, Boyuan Wang, Han Yu, Ming Huang, Jinghong He, Shuanglin Dong, Yangen Zhou, Qinfeng Gao, Yuan Tian   +9 more
doaj   +1 more source

GENETIC CHANGES WITH GENERATIONS OF ARTIFICIAL SELECTION [PDF]

Genetics, 1980
ABSTRACT Using conditional probabilities and moment-generating matrices, I derived approximate algebraic equations that give expectations of gene frequency, population mean, gene frequency variance within lines, or heterozygosity, and gene frequency variance between lines, or drift, for repeated cycles of recurrent selection in ...
openaire   +4 more sources

Genomic Analysis To Identify Signatures of Artificial Selection and Loci Associated with Important Economic Traits in Duroc Pigs

G3: Genes, Genomes, Genetics, 2018
Identifying genetic basis of domestication and improvement in livestock contributes to our understanding of the role of artificial selection in shaping the genome.
Yunlong Ma, Saixian Zhang, Kaili Zhang, Chengchi Fang, Shengsong Xie, Xiaoyong Du, Xinyun Li, D. Ni, Shuhong Zhao   +8 more
semanticscholar   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Artificial Selection and the Marriage Problem [PDF]

Monist, 1891
n ...
openaire   +1 more source

Resequencing of cv CRI‐12 family reveals haplotype block inheritance and recombination of agronomically important genes in artificial selection

Plant Biotechnology Journal, 2018
Summary Although efforts have been taken to exploit diversity for yield and quality improvements, limited progress on using beneficial alleles in domesticated and undomesticated cotton varieties is limited.
Xuke Lu, Xiaoqiong Fu, Delong Wang, Junyi Wang, Xiugui Chen, Meirong Hao, Junjuan Wang, Kyle A. Gervers, Lixue Guo, Shuai Wang, Z. Yin, W. Fan, C. Shi, Xiaoge Wang, Jun Peng, Chao Chen, Ruifeng Cui, N. Shu, Binglei Zhang, Mingge Han, Xiaojie Zhao, M. Mu, John Z. Yu, W. Ye   +23 more
semanticscholar   +1 more source