Results 81 to 90 of about 4,966,594 (348)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Genomic Insights into Genetic Diversity and Adaptation of Nanyang Cattle: Implications for Conservation and Breeding

Animals
The conservation of agricultural animal resources holds significant importance for addressing future breeding demands and promoting sustainable utilization of regional livestock genetic resources.
Yan Zhang, Xian Liu, Jiakun Liu, Tong Fu, Hetian Huang, Mingpeng Han, Dong Liang, Tengyun Gao   +7 more
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Evolution of Divergent Female Mating Preference in Response to Experimental Sexual Selection [PDF]

, 2014
Sexual selection is predicted to drive the coevolution of mating signals and preferences (mating traits) within populations, and could play a role in speciation if sexual isolation arises due to mating trait divergence between populations. However, few
Debelle, A., Snook, R.R., Snook, Rhonda R., Ritchie, Michael G., Debelle, Allan, Ritchie, M.G.   +5 more
core   +1 more source

Adaptive laboratory evolution enhances cell yield and lipid production of Chlorella sorokiniana under mildly cold conditions

Frontiers in Microbiology
One challenge to large-scale microalgae cultivation, e.g., for biodiesel production, is the seasonal low-temperature conditions. We argue that seasonally varying selection in natural environments has prevented algae from better adapting to cold ...
Shu-Yi Lu, Shi-Yu Zhang, Quan-Guo Zhang
doaj   +1 more source

The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas

Frontiers in Marine Science, 2020
Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal ...
Fangfang Ding, Fangfang Ding, Ao Li, Ao Li, Ao Li, Ao Li, Rihao Cong, Rihao Cong, Xinxing Wang, Xinxing Wang, Wei Wang, Wei Wang, Wei Wang, Huayong Que, Huayong Que, Guofan Zhang, Guofan Zhang, Guofan Zhang, Guofan Zhang, Li Li, Li Li, Li Li, Li Li   +22 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Intraspecific variation in morphological and defensive traits of guava genotypes and their implications for herbivory

Frontiers in Horticulture
IntroductionIntraspecific genotypic variation arising from artificial selection can shift how plants allocate resources, often unbalancing traits related to growth, reproduction, and defense.
Johnattan Hernández-Cumplido, Araceli Romero-Pérez, Cesar Rodriguez-Saona, Elizabeth Quintana-Rodríguez, Domancar Orona-Tamayo, José Saúl Padilla-Ramírez, Alicia Callejas-Chavero, Rodrigo Tapia-Vela   +7 more
doaj   +1 more source

Crop plants as models for understanding plant adaptation and diversification

Frontiers in Plant Science, 2013
Since the time of Darwin, biologists have understood the promise of crop plants and their wild relatives for providing insight into the mechanisms of phenotypic evolution.
Kenneth M Olsen, Jonathan F Wendel
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source