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Development of an Autoinducible Plasmid for Recombinant Protein Production
Protein & Peptide Letters, 2021Background: The production of recombinant proteins in E. coli involves such factors as host strains, expression vectors, culture media, and induction methods. The typical procedure to produce heterologous proteins consists of the following: (1) insertion of the target gene into a suitable vector to construct an overexpression plasmid, (2 ...
Young Kee Chae, Hakbeom Kim
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Peptide autoinducers in bacteria
Microbiology, 2009The review classifies and analyzes the literature data on bacterial peptide autoinducers (AIs), responsible for intra- and interspecies communication (quorum sensing) between bacterial populations. The most important families of peptide AI are discussed, including a large group of bacteriocins, subdivided into lantibiotics (class I), unmodified heat ...
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Structural identification of autoinducer of Photobacterium fischeri luciferase
Biochemistry, 1981Synthesis of bacterial luciferase in some strains of luminous bacteria requires a threshold concentration of an autoinducer synthesized by the bacteria and excreted into the medium. Autoinducer excreted by Photobacterium fischeri strain MJ-1 was isolated from the cell-free medium by extraction with ethyl acetate, evaporation of solvent, workup with ...
A, Eberhard +5 more
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Isolation of agr Quorum Sensing Autoinducers
2010Autoregulation of genes is often associated with quorum sensing systems where bacteria produce and secrete molecules that allow the cells to communicate with one another, leading to the activation of certain genes at certain population densities. Here we describe the identification of the agr as a quorum sensing system in Staphylococcus aureus and the ...
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Convergence of hormones and autoinducers at the host/pathogen interface
Analytical and Bioanalytical Chemistry, 2006Most living organisms possess sophisticated cell-signaling networks in which lipid-based signals modulate biological effects such as cell differentiation, reproduction and immune responses. Acyl homoserine lactone (AHL) autoinducers are fatty acid-based signaling molecules synthesized by several Gram-negative bacteria that are used to coordinate gene ...
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