Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false-positive control [PDF]
, 2010 Proteolytic treatment of intact bacterial cells is an ideal means for identifying surface-exposed peptide epitopes and has potential for the discovery of novel vaccine targets.
Cordwell, Stuart J +2 more
core +1 more source
Zymogram and Preliminary Characterization of Lactobacillus helveticus Autolysins [PDF]
Applied and Environmental Microbiology, 1995 The autolysins of Lactobacillus helveticus ISLC5 were detected and partially characterized by renaturing sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). By using lyophilized Micrococcus luteus cells or heated whole cells of L.
Valence, Florence, Lortal, Sylvie
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Autolysins during sporulation of Bacillus subtilis 168 [PDF]
FEMS Microbiology Letters, 2006 Conditions for zymographic detection of a 41-kDa spore cortex hydrolysis-specific autolysin, A6, from Bacillus subtilis 168 were optimised. A6 was present during sporulation from stages II–IV and remained active in the dormant spore. Its expression was controlled by the mother cell-specific early-sporulation sigma factor σE.
Thomas J Smith, Simon J Foster
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Relevance of cell wall and extracellular matrix proteins to Staphylococcus epidermidis adhesion and biofilm formation [PDF]
, 2009 In the present study, the protein profiles of the cell wall (CW) extracts of eight Staphylococcus epidermidis strains were analysed. The protein pattern of the extracellular matrix (EM) of four S.
Henriques, Mariana +3 more
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Les autolysines des bactéries lactiques [PDF]
Le Lait, 1996 Les autolysines sont definies comme des enzymes bacteriennes endogenes capables d'hydrolyser le peptidoglycane, qui est le constituant majeur et essentiel de la paroi. Leur action peut donc induire la lyse bacterienne. Un certain nombre d'autolysines ont ete caracterisees au niveau moleculaire chez differentes bacteries.
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Protein Complexes and Proteolytic Activation of the Cell Wall Hydrolase RipA Regulate Septal Resolution in Mycobacteria [PDF]
, 2013 Peptidoglycan hydrolases are a double-edged sword. They are required for normal cell division, but when dysregulated can become autolysins lethal to bacteria.
Alber, Tom +7 more
core +3 more sources
Characterization of LysBC17, a Lytic Endopeptidase from Bacillus cereus
Antibiotics, 2019 Bacillus cereus, a Gram-positive bacterium, is an agent of food poisoning. B. cereus is closely related to Bacillus anthracis, a deadly pathogen for humans, and Bacillus thuringenesis, an insect pathogen.
Steven M. Swift +6 more
doaj +1 more source
Comparison of the action of different proteases on virulence properties related to the staphylococcal surface. [PDF]
, 2012 AIMS: The purpose of this study was to evaluate the antimicrobial efficacy of five different proteases belonging to two different families on Staphylococcus aureus and Staphylococcus epidermidis strains.
G. Barbato +6 more
core +1 more source
Aqueous enzymatic protein and lipid release from the microalgae Chlamydomonas reinhardtii
Bioresources and Bioprocessing, 2020 Advances in biochemical and molecular manipulation have led to increased biomass productivity and oil accumulation in the microalgae C. reinhardtii. However, scalable processes for the recovery of oil and other valuable biomolecules, such as protein ...
Laura Soto-Sierra +2 more
doaj +1 more source
Contribution of autolysin to virulence of Streptococcus pneumoniae [PDF]
Infection and Immunity, 1989 Insertion-duplication mutagenesis was used to construct an autolysin-negative derivative of Streptococcus pneumoniae. This derivative was obtained by first transforming the nonencapsulated strain Rx1 with a derivative of the vector pVA891 carrying a 375-base-pair TaqI DNA fragment from the middle of the autolysin structural gene. DNA was extracted from
A M, Berry +3 more
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