Results 201 to 210 of about 17,929 (235)

Kinesin 1 Drives Autolysosome Tubulation [PDF]

Developmental Cell, 2016
Autophagic lysosome reformation (ALR) plays an important role in maintaining lysosome homeostasis. During ALR, lysosomes are reformed by recycling lysosomal components from autolysosomes. The most noticeable step of ALR is autolysosome tubulation, but it is currently unknown how the process is regulated.
Qian Peter Su, Yang Chen, Yueyao Zhu
exaly   +3 more sources

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Autophagosomal components recycling on autolysosomes

Trends in Cell Biology, 2022
Autophagy is a multistage, intracellular process. Here, we highlight a recently identified autophagosomal components recycling (ACR) stage and the recycler complex (SNX4-SNX5-SNX17), which mediates recycling of autophagosomal outer membrane proteins on the autolysosome surface immediately following autophagosome-lysosome fusion.
Yufen Wang, Huilin Que, Yueguang Rong
openaire   +2 more sources

Migratory autolysosome disposal mitigates lysosome damage

Journal of Cell Biology
Lysosomes, essential for intracellular degradation and recycling, employ damage-control strategies such as lysophagy and membrane repair mechanisms to maintain functionality and cellular homeostasis. Our study unveils migratory autolysosome disposal (MAD), a response to lysosomal damage where cells expel LAMP1-LC3 positive structures via autolysosome ...
Takami Sho, Ying Li, Haifeng Jiao
exaly   +3 more sources

RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells [PDF]

Autophagy, 2019
Multiple sources contribute membrane and protein machineries to construct functional macroautophagic/autophagic structures. However, the underlying molecular mechanisms remain elusive. Here, we show that RAB2 connects the Golgi network to autophagy pathway by delivering membrane and by sequentially engaging distinct autophagy machineries. In unstressed
Xianming Ding, Xiao Jiang, Rui Tian
exaly   +3 more sources

Recycling of autophagosomal components from autolysosomes by the recycler complex

Nature Cell Biology, 2022
Autolysosomes contain components from autophagosomes and lysosomes. The contents inside the autolysosomal lumen are degraded during autophagy, while the fate of autophagosomal components on the autolysosomal membrane remains unknown. Here we report that the autophagosomal membrane components are not degraded, but recycled from autolysosomes through a ...
Chuchu Zhou, Zhe Wu, Wanqing Du, Huilin Que, Yufen Wang, Qinqin Ouyang, Fenglei Jian, Weigang Yuan, Yuan Zhao, Rui Tian, Ying Li, Yang Chen, Shuaixin Gao, Catherine C. L. Wong, Yueguang Rong   +14 more
openaire   +2 more sources

Dissecting the dynamic turnover of GFP-LC3 in the autolysosome [PDF]

Autophagy, 2011
Determination of autophagic flux is essential to assess and differentiate between the induction or suppression of autophagy. Western blot analysis for free GFP fragments resulting from the degradation of GFP-LC3 within the autolysosome has been proposed as one of the autophagic flux assays.
Hong-Min Ni, Xiao-Ming Yin, Wen-Xing Ding   +2 more
exaly   +3 more sources

Oleic acid-induced defective autolysosome shows impaired lipid degradation

Biochemical and Biophysical Research Communications, 2019
Recent studies suggest an alternative pathway of lipid breakdown called lipophagy, which delivers lipid droplets (LDs) to lysosomes for degradation of LDs. However, molecular mechanisms regulating lipophagy are still largely unknown. In this study, we evaluated the effect of oleic acid (OA) on lipophagy in cells.
Da-Hye Lee, Jiyun Ahn, Young Jin Jang, Tae-Youl Ha, Chang Hwa Jung   +4 more
openaire   +2 more sources

Appearance of Autolysosomes in Rat Liver after Leupeptin Treatment

The Journal of Biochemistry, 1982
The administration to rats of leupeptin produced prominent numbers of enlarged and irregularly shaped autolysosomes in hepatocytes. Percoll density equilibration of crude lysosomal fractions from rat livers showed that most lysosomal enzyme migrated form normal lysosomal density fractions toward higher density fractions within 30 min after leupeptin ...
K, Furuno, T, Ishikawa, K, Kato
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Isolation of Autolysosomes from Tobacco BY-2 Cells

2016
Autolysosomes are organelles that sequester and degrade a portion of the cytoplasm during autophagy. Although autophagosomes are short lived compared to other organelles such as mitochondria, plastids, and peroxisomes, many autolysosomes accumulate in tobacco BY-2 cells cultured under sucrose starvation conditions in the presence of a cysteine protease
Chihiro, Takatsuka, Yuko, Inoue-Aono, Yuji, Moriyasu   +2 more
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Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation

Journal of Cell Science, 2018
ABSTRACT Macroautophagy (simply called autophagy hereafter) is an intracellular degradation mechanism that is activated by nutrient starvation. Although it is well known that starvation induces autophagosome formation in an mTORC1-dependent manner, whether starvation also regulates autophagosome or autolysosome maturation was unclear. In
Yuta Homma, Naonobu Fujita, Mitsunori Fukuda   +2 more
exaly   +3 more sources