Results 21 to 30 of about 62,100 (242)
Short and long sleeping mutants reveal links between sleep and macroautophagy
eLife, 2021 Sleep is a conserved and essential behavior, but its mechanistic and functional underpinnings remain poorly defined. Through unbiased genetic screening in Drosophila, we discovered a novel short-sleep mutant we named argus.
Joseph L Bedont +7 more
doaj +1 more source
Scission, a critical step in autophagosome formation. [PDF]
Autophagy, 2020 A key feature of macroautophagy (hereafter autophagy) is the formation of the phagophore, a double-membrane compartment sequestering cargos and finally maturing into a vesicle termed an autophagosome; however, where these membranes originate from is not clear.
Lei Y, Klionsky DJ.
europepmc +4 more sources
Membrane closure in stress induced-autophagosome formation [PDF]
Cell Stress, 2018 Eiji Morita
doaj +3 more sources
Membrane dynamics of ATG4B and LC3 in autophagosome formation. [PDF]
J Mol Cell Biol, 2022 Abstract The biogenesis of autophagosomes provides the basis for macroautophagy to capture and degrade intracellular cargoes. Binding of the autophagy-related protein ATG8/LC3 to autophagic membranes is essential to autophagosome formation, which involves the specific and dynamic processing of ATG8/LC3 by cysteine protease ATG4. However,
Zhou Y +7 more
europepmc +4 more sources
An initial step of GAS-containing autophagosome-like vacuoles formation requires Rab7. [PDF]
PLoS Pathogens, 2009 Group A streptococcus (GAS; Streptococcus pyogenes) is a common pathogen that invades non-phagocytic human cells via endocytosis. Once taken up by cells, it escapes from the endocytic pathway to the cytoplasm, but here it is contained within a membrane ...
Hitomi Yamaguchi +5 more
doaj +1 more source
Calpain as a Novel Regulator of Autophagosome Formation [PDF]
Autophagy, 2007 Ubiquitously expressed micro- and m-calpain proteases consist of 80-kDa catalytic subunits encoded by the Capn1 and Capn2 genes, respectively, and a common 28-kDa regulatory subunit encoded by the calpain small 1 (Capns1) gene. The micro- and m-calpain proteases have been implicated in both pro- or anti-apoptotic functions.
DEMARCHI F +4 more
openaire +3 more sources
Biology Open, 2017 Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure,
Yuta Ogasawara +4 more
doaj +1 more source
Arabidopsis NAP1 Regulates the Formation of Autophagosomes [PDF]
Current Biology, 2016 The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1, 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluorescent protein fusions, we have found that NAP1 [4], a component of the SCAR/WAVE complex, locates to ...
Wang, P. +3 more
openaire +4 more sources
Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. [PDF]
PLoS ONE, 2016 Autophagy acts as a host-defense system against pathogenic microorganisms such as Group A Streptococcus (GAS). Autophagy is a membrane-mediated degradation system that is regulated by intracellular membrane trafficking regulators, including small GTPase ...
Seiichiro Oda +6 more
doaj +1 more source
Origin of the Autophagosomal Membrane in Plants
Frontiers in Plant Science, 2016 During autophagy, cargo molecules destined for degradation are sequestrated into a double membrane structure called autophagosome, which subsequently fuses with the vacuole.
Xiaohong Zhuang +3 more
doaj +1 more source