Autophagosome Formation: Tracing the Source [PDF]
Developmental Cell, 2013 Although it is well appreciated that autophagy begins with phagophore formation and expansion through lipid acquisition to become the autophagosome, this process remains poorly understood, with the source of autophagosome membrane controversial. Reporting recently in Nature, Hamasaki et al.
Bernard, Amélie, Klionsky, Daniel J.
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Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells. [PDF]
PLoS ONE, 2013 Mycobacterium tuberculosis is an intracellular pathogen that can survive within phagocytic cells by inhibiting phagolysosome biogenesis. However, host cells can control the intracellular M. tuberculosis burden by the induction of autophagy. The mechanism
Shintaro Seto +3 more
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Short and long sleeping mutants reveal links between sleep and macroautophagy
eLife, 2021 Sleep is a conserved and essential behavior, but its mechanistic and functional underpinnings remain poorly defined. Through unbiased genetic screening in Drosophila, we discovered a novel short-sleep mutant we named argus.
Joseph L Bedont +7 more
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ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation.
Molecules and Cells, 2022 ATG9A and ATG2A are essential core members of the autophagy machinery. ATG9A is a lipid scramblase that allows equilibration of lipids across a membrane bilayer, whereas ATG2A facilitates lipid flow between tethered membranes.
A. V. van Vliet +14 more
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Membrane closure in stress induced-autophagosome formation [PDF]
Cell Stress, 2018 Eiji Morita
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Scission, a critical step in autophagosome formation. [PDF]
Autophagy, 2020 A key feature of macroautophagy (hereafter autophagy) is the formation of the phagophore, a double-membrane compartment sequestering cargos and finally maturing into a vesicle termed an autophagosome; however, where these membranes originate from is not clear.
Lei Y, Klionsky DJ.
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Structure, lipid scrambling activity and role in autophagosome formation of ATG9A. [PDF]
Nat Struct Mol Biol, 2020 De novo formation of the double-membrane compartment autophagosome is seeded by small vesicles carrying membrane protein autophagy-related 9 (ATG9), the function of which remains unknown.
Maeda S +9 more
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Calpain as a Novel Regulator of Autophagosome Formation [PDF]
Autophagy, 2007 Ubiquitously expressed micro- and m-calpain proteases consist of 80-kDa catalytic subunits encoded by the Capn1 and Capn2 genes, respectively, and a common 28-kDa regulatory subunit encoded by the calpain small 1 (Capns1) gene. The micro- and m-calpain proteases have been implicated in both pro- or anti-apoptotic functions.
DEMARCHI F +4 more
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Biology Open, 2017 Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure,
Yuta Ogasawara +4 more
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Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. [PDF]
PLoS ONE, 2016 Autophagy acts as a host-defense system against pathogenic microorganisms such as Group A Streptococcus (GAS). Autophagy is a membrane-mediated degradation system that is regulated by intracellular membrane trafficking regulators, including small GTPase ...
Seiichiro Oda +6 more
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