Results 91 to 100 of about 58,150 (299)

Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, EarlyView.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

Electron Microscopy of autophagosomes and autolysosomes in cells expressing different levels of DRD3.

, 2016
(A) Representative electron microscopic images of two autophagosomes (AP, left panel) and an autolysosome (AL, right panel). Scale bar: 500 nm. (B) Quantification of autophagosomes and autolysosomes in CHO cells and CHO cells overexpressing GFP-DRD3-Flag.
Xin Zhang (35492), Qingsong Liu (381059), Zhiyuan Li (205620), Wenchao Wang (242659), Ulrike S. Eggert (2041642), Juanjuan Liu (384099), Xiaofei Liang (843086), Hong Wu (105254), Jing Liu (38537), Xinmiao Ji (3173772)   +9 more
core   +1 more source

Decoding the Mechanism of Action of a Parasite TGFβ Antagonist Inspires the Creation of Cell‐Type‐Specific TGFβ Modulators

Advanced Science, EarlyView.
The mammalian TGFβ interacts with ubiquitously expressed TGFBR1 and TGFBR2, and current TGFβ‐targeting agents are non‐cell‐selective. The cooperative interaction of the modular parasite TGFβ antagonist with multiple host (co‐)receptors empowers the design of TGM chimeras and bispecific antibodies that activate or inhibit TGFβ signaling in a cell ...
Maarten van Dinther, Tristin Schwartze, Jiying Zhang, Kun Fan, Gerard van der Zon, Luke Power, Cynthia S. Hinck, Claire Ciancia, Ananya Mukundan, Roman Gonzalez‐Prieto, Peter van Veelen, Rick M. Maizels, Andrew P. Hinck, Peter ten Dijke   +13 more
wiley   +1 more source

Autophagosome maturation in primary rat hepatocytes.

, 2006
Nutrient deprivation of eukaryotic cells elicits a rapid survival response, including the induction of autophagy. Autophagy, or "self-eating", involves the formation of autophagosomes from an unknown membrane source and the sequestration of cytosolic ...
Kchl, R., Köchl, Robert
core  

Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery [PDF]

, 2013
Cationic liposome (lipoplex) and polymer (polyplex)-based vectors have been developed for nonviral gene delivery. These vectors bind DNA and enter cells via endosomes, but intracellular transfer of DNA to the nucleus is inefficient.
Roberts, Rebecca, Kostarelos, K, Kostarelos, Kostas, Wileman, Thomas, Jefferson, Matthew, Thomas, Paul, Jefferson, M, van den Bossche, J, Al-Jamal, Wafa, Powell, Penny P., Powell, PP, Wileman, T, Powell, Penny P, Thomas, P, Whelband, Matthew, Roberts, R, Wileman, Tom, van den Bossche, Jeroen, Al-Jamal, WT, Whelband, M, Al-Jamal, Wafa T., Al-Jamal, Wafa' T   +21 more
core   +1 more source

A Biomimetic Nanoparticle System Intercepts and Degrades Thrombospondin‐1 to Restore Vascular Homeostasis After Ischemic Injury

Advanced Science, EarlyView.
TAX2‐NPs capture extracellular TSP‐1 in the injured liver and promote its macrophage‐mediated autophagic degradation. This process blocks TSP‐1/CD47 signaling, restores VEGFR2‐AKT activity, preserves endothelial function, and mitigates hepatic ischemia‐reperfusion injury.
Haorui Wang, Xiandi Meng, Yanbao Xin, Kuirong Mao, Huizhu Tan, Changkai Ma, Xiuxiu Cong, Mengfei Zhao, Meiling Yu, Si Chen, Yue Hou, Yong‐Guang Yang, Tianmeng Sun   +12 more
wiley   +1 more source

Abnormal autophagosomes in 15-month-old 3xTg-AD mice.

, 2013
Electron microscope sections obtained from CA1 regions of 15-month-old 3xTg-AD mice. Sections show examples of enlarged autophagosomes containing electron-dense undigested materials.
Salvatore Oddo (212099), Randy Strong (181994), Arlan Richardson (194869), Smita Majumder (343250)   +3 more
core   +1 more source

Targeting Endogenous Lipophagy: A Novel Strategy to Enhance MSC Osteogenesis and Mineralization for Senile Osteoporosis Therapy

Advanced Science, EarlyView.
Schematic representation of the role of lipophagy in bone mesenchymal stem cells(MSCs). In healthy MSCs, functional lipophagy efficiently degrades lipid droplets to support oxidative phosphorylation and cellular energy production, thereby facilitating osteogenic differentiation and matrix mineralization.
Chaoqiang Chen, Zhidong Liu, Yanhang Sun, Xiaojun Xu, Zhexiao Lan, Junhao Zhao, Peitao Xu, Guiwen Ye, Jinteng Li   +8 more
wiley   +1 more source

NET‐DNA Activates the ANXA2/TMEM215/BiP Axis to Promote Mitophagy‐Mediated Anoikis Resistance in Endometriosis

Advanced Science, EarlyView.
NET‐DNA is enriched during early endometriotic lesion establishment and drives anoikis resistance by promoting an ANXA2–TMEM215–BiP axis. This pathway strengthens ER–mitochondria contacts, enhances PINK1/Parkin‐associated mitophagy, preserves mitochondrial homeostasis, and supports ectopic stromal cell survival, thereby facilitating lesion persistence ...
Honglin Wang, Yanling Gou, Huiyan Zhang, Hongli Wang, Beidi Wang, Jinming Liu, Yingying Cao, Ruru Bai, Yuxin Zhao, Xu Han, Chao Feng, Xin Huang, Zongfeng Zhang   +12 more
wiley   +1 more source

Making an autophagosome [PDF]

The Journal of Cell Biology, 2006
An understanding of de novo organelle construction comes one step closer thanks to He et al. (page [925][1]), who find that Atg11 leads Atg9 to the preautophagosomal structure (PAS). The PAS is intriguing because it is the site where fragments of membrane coalesce to form a new organelle ...
openaire   +1 more source