Results 111 to 120 of about 8,766 (148)
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The NP Activation Test for Assay of Avian Leukosis/Sarcoma Viruses
Avian Diseases, 1970SUMMARY Procedures are described for routine isolation and production of nonproducing Rous sarcoma cells (NP cells) and for use of these cells in the NP activation test for assay of avian leukosis/sarcoma viruses. The NP activation test was found to be sensitive, reproducible, easy to perform, and highly reliable.
B H, Rispens +3 more
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Phenotypic mixing between reticuloendotheliosis virus and avian sarcoma viruses
Virology, 1977Abstract Coinfection of chicken embryo fibroblasts with reticuloendotheliosis virus (REV) and avian sarcoma virus leads to the formation of avian sarcoma viral pseudotypes which carry envelope determinants of REV. These pseudotypes can be neutralized by REV antiserum, have a host range which is different from that of any known avian sarcoma virus ...
P K, Vogt +4 more
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The origin of 7 S RNA in virions of avian sarcoma viruses
Virology, 1976Abstract Duck and chick embryo fibroblasts were labeled with 32 PO 4 3− and cellular 7S RNA was purified. Ribonuclease T 1 digests were prepared and the resulting oligonucleotides were fractionated by two-dimensional electrophoresis. Duck 7 S RNA lacked one large oligonucleotide present in chicken 7 S RNA, thus establishing that the two species ...
E, Erikson, R L, Erikson
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Ultraviolet inactivation of avian sarcoma viruses: Biological and biochemical analysis
Virology, 1976Abstract The rate of inactivation by ultraviolet light of the focus-forming capacity of avian sarcoma virus was almost the same as that of the virus-producing capacity, measured as plaque formation. In addition, no significant difference was observed in inactivation of the transforming capacity assayed on C/BE chick embryo fibroblasts (CEF), which ...
M, Owada +4 more
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Sensitivity to γ rays of avian sarcoma and murine leukemia viruses
Virology, 1980Abstract The direct inactivation of avian and murine oncoviruses by γ rays was examined using 60 Co as a γ-ray source. The inactivation of murine leukemia virus (M-MuLV) followed single-hit kinetics with a target size comparable to the molecular weight of its genomic RNA.
K, Toyoshima +5 more
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International Journal of Cancer, 1972
AbstractClonal lines of murine and avion sarcoma virus‐transformed mammalian cells, in which the viruses do not productively replicate, were studied for the occurrence of spontaneous or chemical‐induced morphologic reversion. From over 10,000 single‐cellderived clones of MSV‐transformed nonproducer cells examined, only one morphologic revertant was ...
Stephenson, J R +2 more
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AbstractClonal lines of murine and avion sarcoma virus‐transformed mammalian cells, in which the viruses do not productively replicate, were studied for the occurrence of spontaneous or chemical‐induced morphologic reversion. From over 10,000 single‐cellderived clones of MSV‐transformed nonproducer cells examined, only one morphologic revertant was ...
Stephenson, J R +2 more
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Cytoskeletal changes induced by two avian sarcoma viruses: UR2 and Rous Sarcoma Virus
Virology, 1984UR2-transformed cells were examined by immunofluorescence and compared to control cells and cells transformed by Rous Sarcoma Virus (RSV). Actin and tubulin which are normally depolymerized in RSV-transformed cells appeared to be unaffected by UR2 transformation.
M F, Notter, P C, Balduzzi
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Abortive infection of Japanese quail cells with avian sarcoma viruses
Virology, 1972Abstract The replication of Prague strains of Rous sarcoma virus (PR RSV) and avian sarcoma virus B77 in Japanese quail cells is abortive in a quantitative sense. Although efficient infection of quail cells, as evidenced by transformation, was obtained with these viruses, the yield of progeny was suppressed by 102-fold or more relative to the chick ...
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[32] Biological techniques for avian sarcoma viruses
1979Publisher Summary This chapter describes a quantitative and reproducible assay of Rous sarcoma virus (RSV), the focus assay, and methods for obtaining cloned preparations of RSV, through both the focus and soft agar-colony assays. Large-scale growth of RSV is discussed in the chapter.
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Surface Alterations in Cells Infected by Avian Leukosis-Sarcoma Viruses
1976The regulatory mechanisms involved in morphogenesis and in the subsequent maintenance of the differentiated state are poorly understood. However, it is clear that ontogenesis requires that cell proliferation and differentiation be strictly controlled to ensure appropriate development of the organism. If we accept hypothetically that it is primarily the
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