Results 281 to 290 of about 136,886 (329)

(Strept)avidin Binds Glycoconjugates

Biochemistry (Moscow)
Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6-O-sulfated ...
Nadezhda V, Shilova, Svetlana M, Polyakova, Alexey Yu, Nokel, Alexander D, Lipatnikov, Elena A, Gordeeva, Marina V, Lavrenteva, Nicolai V, Bovin   +6 more
openaire   +2 more sources

Avidin-Biotin Technology

2007
absent
Françoise Nau, Catherine Guérin-Dubiard, Marc Croguennec   +2 more
openaire   +3 more sources

Avidine

2007
absent
Nau, Francoise, Guérin-Dubiard, Catherine, Croguennec, Thomas   +2 more
openaire   +1 more source

Genetically engineered avidins and streptavidins

Cellular and Molecular Life Sciences, 2006
Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept ...
O H, Laitinen, V P, Hytönen, H R, Nordlund, M S, Kulomaa   +3 more
openaire   +2 more sources

[6] Nonglycosylated avidin

1990
Publisher Summary This chapter describes a method for the isolation of the non-glycosylated tetramer. This method is based on the removal of glycosylated forms using appropriate lectin columns. Belovo avidin is dissolved in 192 ml distilled water and the solution is centrifuged. The clarified solution is applied to a Con A column. Tris buffer is used
Yaffa Hiller, Edward A. Bayer, Meir Wilchek   +2 more
openaire   +1 more source

Towards a synthetic avidin mimic

Analytical and Bioanalytical Chemistry, 2011
A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine.
Jesper, Wiklander, Björn C G, Karlsson, Teodor, Aastrup, Ian A, Nicholls   +3 more
openaire   +2 more sources

Avidin Binds to Condensed Chromatin

Stain Technology, 1977
The protein avidin has been conjugated with fluorescein isothiocyanate and then used to treat cells and chromosome preparations. Fluorescence microscopy reveals that this protein is bound by areas of the specimen containing condensed chromatin.
openaire   +2 more sources

The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods

2003
Immunoenzyme methods can be enhanced by the use of the high affinity molecules, avidin and biotin. The binding of avidin to biotin is almost irreversible. By labeling a detection enzyme such as horseradish peroxidase with biotin, and a secondary antibody (reactive against the antigen detecting primary antibody) with biotin as well, these two compounds ...
openaire   +3 more sources

Biotin–Avidin System-Based Delivery Enhances the Therapeutic Performance of MSC-Derived Exosomes

ACS Nano, 2023
Daokun Deng, Fa-Ming Chen, Xiao-Tao He
exaly  

The avidin-biotin system

Immunology Today, 1984
R E, Morris, C B, Saelinger
openaire   +2 more sources