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Behavior of avidin and avidin/bisbiotin polymers at the air-water interface
Journal of Colloid and Interface Science, 1991Abstract The surface behavior of avidin and two polymers, formed by affinity polymerization from two different stoichiometric mixtures of avidin and the bifunctional ligand bisbiotin, has been investigated at the air/water interface. Strong hysteresis in the pressure-area isotherms is attributed to mechanical distortion of the molecules.
Claudius D'Silva +2 more
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Towards a synthetic avidin mimic
Analytical and Bioanalytical Chemistry, 2011A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine.
Björn Karlsson +4 more
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Avidin Binds to Condensed Chromatin
Stain Technology, 1977The protein avidin has been conjugated with fluorescein isothiocyanate and then used to treat cells and chromosome preparations. Fluorescence microscopy reveals that this protein is bound by areas of the specimen containing condensed chromatin.
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Dual‐affinity avidin molecules
Proteins: Structure, Function, and Bioinformatics, 2005AbstractA recently reported dual‐chain avidin was modified further to contain two distinct, independent types of ligand‐binding sites within a single polypeptide chain. Chicken avidin is normally a tetrameric glycoprotein that binds water‐soluble d‐biotin with extreme affinity (Kd ≈ 10−15 M). Avidin is utilized in various applications and techniques in
Olli H. Laitinen +10 more
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Hepatocyte targeting of 111In-labeled oligo-DNA with avidin or avidin–dendrimer complex
Journal of Controlled Release, 2004To establish an effective nonviral gene transfer vector to hepatocytes, various oligo-carrier complexes were developed employing dendrimer (G4) and avidin-biotin systems (Av-bt), and their biodistribution were evaluated. In-111-labeled-oligo, without any carriers, showed low uptake in normal organs other than the kidney (21.48% ID/g at 15 min, 18.48 ...
Takayoshi Ishimori +7 more
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Avidin-Biotin Immobilisation Systems
1993Abstract Over the past two decades, the avid in–biotin system has evolved to be an extremely useful tool in the fields of biology, biochemistry, and biotechnology. The extraordinary affinity (Kd ∼ 10−15 M) of avid in (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system.
M. Wilchek, E. A. Bayer
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1990
Publisher Summary This chapter describes a method for the isolation of the non-glycosylated tetramer. This method is based on the removal of glycosylated forms using appropriate lectin columns. Belovo avidin is dissolved in 192 ml distilled water and the solution is centrifuged. The clarified solution is applied to a Con A column. Tris buffer is used
Meir Wilchek +2 more
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Publisher Summary This chapter describes a method for the isolation of the non-glycosylated tetramer. This method is based on the removal of glycosylated forms using appropriate lectin columns. Belovo avidin is dissolved in 192 ml distilled water and the solution is centrifuged. The clarified solution is applied to a Con A column. Tris buffer is used
Meir Wilchek +2 more
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The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods [PDF]
, 2003 Immunoenzyme methods can be enhanced by the use of the high affinity molecules, avidin and biotin. The binding of avidin to biotin is almost irreversible. By labeling a detection enzyme such as horseradish peroxidase with biotin, and a secondary antibody (reactive against the antigen detecting primary antibody) with biotin as well, these two compounds ...
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1979
Publisher Summary This chapter presents an overview of the microassay of avidin. Avidin is a biotin-binding protein first detected in the egg white of the hen and later in the avian and amphibian oviduct. The study of avidin induction is extensive because avidin induction by progesterone in the chick oviduct is one of the best models for study of the
Pentti Tuohimaa, Heikki A. Elo
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Publisher Summary This chapter presents an overview of the microassay of avidin. Avidin is a biotin-binding protein first detected in the egg white of the hen and later in the avian and amphibian oviduct. The study of avidin induction is extensive because avidin induction by progesterone in the chick oviduct is one of the best models for study of the
Pentti Tuohimaa, Heikki A. Elo
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