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Avidin and streptavidin in clinical diagnostics
Biomolecular Engineering, 1999With the introduction of the SA/Bi technology into the clinical diagnostic field ultrasensitive tests with an acceptable turn-around time became feasible. Cytokine ELISAs with lower detection limit in the picogram range became finally available. Prior to the introduction of SA/Bi only elevated levels of target could be detected by short time tests but ...
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(Strept)avidin Binds Glycoconjugates
Biochemistry (Moscow)Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6-O-sulfated ...
Nadezhda V, Shilova +6 more
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Biotin–Avidin System-Based Delivery Enhances the Therapeutic Performance of MSC-Derived Exosomes
ACS Nano, 2023Daokun Deng, Fa-Ming Chen, Xiao-Tao He
exaly
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
2003Immunoenzyme methods can be enhanced by the use of the high affinity molecules, avidin and biotin. The binding of avidin to biotin is almost irreversible. By labeling a detection enzyme such as horseradish peroxidase with biotin, and a secondary antibody (reactive against the antigen detecting primary antibody) with biotin as well, these two compounds ...
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The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis
Journal of Controlled Release, 2017, Kun Cheng
exaly

