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[20] Monovalent avidin affinity columns
1990Publisher Summary Monovalent and tetravalent avidin affinity columns retain biotin-containing compounds. Because the affinity for biotin is lower with avidin monomers than with avidin tetramers, two columns containing these reagents are known as avidin monomer affinity columns. Tetrameric avidin is first coupled to cyanogen bromide activated agarose.
R. A. Kohanski, M. D. Lane
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Avidin and streptavidin in clinical diagnostics
Biomolecular Engineering, 1999With the introduction of the SA/Bi technology into the clinical diagnostic field ultrasensitive tests with an acceptable turn-around time became feasible. Cytokine ELISAs with lower detection limit in the picogram range became finally available. Prior to the introduction of SA/Bi only elevated levels of target could be detected by short time tests but ...
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1970
Publisher Summary In addition to microbiological methods, which are extremely sensitive but require time-consuming manipulation for assay of avidin, several new methods have been developed recently in various laboratories. Details of three simple and reliable assays are given in this chapter.
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Publisher Summary In addition to microbiological methods, which are extremely sensitive but require time-consuming manipulation for assay of avidin, several new methods have been developed recently in various laboratories. Details of three simple and reliable assays are given in this chapter.
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1970
Publisher Summary The chapter describes the method for the purification of avidin, which is a very stable, water-soluble glycoprotein. Avidin has been purified from egg white, freshly separated or frozen, by a number of different procedures, most of them based on an initial adsorption step to separate it from the bulk of the other egg white proteins.
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Publisher Summary The chapter describes the method for the purification of avidin, which is a very stable, water-soluble glycoprotein. Avidin has been purified from egg white, freshly separated or frozen, by a number of different procedures, most of them based on an initial adsorption step to separate it from the bulk of the other egg white proteins.
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A Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin
Biochemical Journal, 1965openaire +3 more sources
The Relationship of Lysozyme, Biotin and Avidin
Science, 1945Gordon Alderton+2 more
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