Results 191 to 200 of about 227,214 (300)

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

Atroposelective interrupted CuAAC reaction using cyclic diaryliodoniums. [PDF]

Nat Commun
Li Y, Yang S, Duan L, Gu Z.
europepmc   +1 more source

High Yield Branched Puromycin Linker Design Enables Efficient cDNA Display and Chemical Modification of Peptides

Biotechnology and Bioengineering, EarlyView.
A novel puromycin linker for cDNA display was synthesized via SPAAC reaction. Its functionality was confirmed by recovering intact DNA after selection against EpCAM. The system also enabled the verification of bicyclic peptides during display, demonstrating its utility for constructing and screening structurally complex peptide libraries.
Simon Schneider, Melanie Boll, Matthias Eder, Ann‐Christin Eder   +3 more
wiley   +1 more source

Influence of intramolecular interactions and transition state energetics on the strain-promoted azide-alkyne cycloaddition of 2-aminobenzenesulfonamide-containing cyclononynes. [PDF]

RSC Adv
Nakahara K, Kaneda K.
europepmc   +1 more source

Genetic code expansion for chemosensing and chemical actuation of biological functions

Bulletin of the Korean Chemical Society, EarlyView.
Genetic code expansion (GCE) broadens the chemical repertoire for precise control of protein function in biological systems. This review highlights GCE‐based sensing and actuating systems that operate through selective interactions between noncanonical amino acids and chemical triggers.
Jieun Bae, Dong‐Hyun Kim, Minseob Koh
wiley   +1 more source

An antibody uniquely binding short 2'-O-methyl RNA oligonucleotide duplexes: formation and recognition of target duplexes on cell surfaces. [PDF]

Front Immunol
Dunn IS, Lawler MM, Fagundes L, Simoes Junior M, Mangold A, Rinaldi D, Rose LB, Kurnick JT.   +7 more
europepmc   +1 more source

Polarization anisotropy identifies bound azide in photoexcited ferric myoglobin: Resolving photodeligation ambiguity by femtosecond infrared spectroscopy

Bulletin of the Korean Chemical Society, EarlyView.
Polarization‐resolved femtosecond TRIR reveals that the transient absorption in MbN3 arises from a bound azide ligand, not photodissociation. Identical anisotropy confirms preserved orientation, demonstrating that Q‐band excitation leads to thermal relaxation without photodeligation, clarifying the photophysics of anion‐bound ferric heme proteins ...
Seongchul Park, Juhyang Shin, Manho Lim
wiley   +1 more source

Spatial Multiomics Combining Lipids and Gene Expression Using MALDI ISH MSI. [PDF]

Anal Chem
Vanderschoot KA, Padilla JP, Steineman KA, De Caro CM, Wang Y, Wang A, Heffern MC, Neumann EK.   +7 more
europepmc   +1 more source

Real time in vivo efficacy monitoring of desialylation and perforation enhanced immunotherapy of cancer

BMEMat, EarlyView.
A multifunctional nanomedicine is designed to engineer NK cells for enhanced cancer immunotherapy through perforation and desialylation, enabling real‐time monitoring of ATP and GrB levels for precise efficacy assessment. Abstract Efficient enhanced NK cell therapy is of critical importance in the immune treatment of cancer.
Yuanjiao Yang, Yuru Wang, Xuemei Li, Chaoyi Yang, Yuhui Yang, Yanyun Fang, Lin Ding, Yunlong Chen, Huangxian Ju   +8 more
wiley   +1 more source

Harnessing oligonucleotide architecture for potent multivalent inhibition of human carbonic anhydrases. [PDF]

RSC Med Chem
Abbass EM, Ladu F, Mansot J, Noël M, Nocentini A, Sechi M, Supuran CT, Smietana M, Winum JY.   +8 more
europepmc   +1 more source