Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site. [PDF]
J Clin Microbiol, 2004 Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.
Vielemeyer O +3 more
europepmc +6 more sources
Do not let perfect be the enemy of good: the current reality of Bordetella testing [PDF]
Microbiology SpectrumDiagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al.
Rebecca Yee
doaj +2 more sources
Comparison of Bordetella species identification among differing rt-PCR assays in the United States [PDF]
Microbiology SpectrumIn the United States, the general laboratory method for diagnosing pertussis, caused by Bordetella pertussis, is real-time PCR (rt-PCR) targeting insertion sequence 481 (IS481).
Matthew Cole +7 more
doaj +2 more sources
Looking beyond Pertussis in Prolonged Cough Illness of Young Children [PDF]
Vaccines, 2022 Pertussis, commonly known as whooping cough, is one of the most poorly controlled vaccine-preventable diseases in the world. South-East Asia is estimated to contribute the most to childhood disease burden while this remains largely unexplored in India ...
Rajlakshmi Viswanathan +6 more
doaj +2 more sources
Bordetella Holmesii: An Unusual Cause of Endogenous Endophthalmitis in a Patient With Sickle Cell Disease. [PDF]
J Vitreoretin Dis, 2021 Purpose: This case report describes a rare organism causing endogenous endophthalmitis in a patient with sickle cell disease. Methods: A case report was conducted.
Pegany RB, George RT, Zhang AY.
europepmc +2 more sources
Bordetella holmesii Lipopolysaccharide Hide and Seek Game with Pertussis: Structural Analysis of the O-Specific Polysaccharide and the Core Oligosaccharide of the Type Strain ATCC 51541. [PDF]
Int J Mol Sci, 2020 Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis, but it also comprises of a pertussis-like illness caused by B. holmesii. The virulence factors of B.
Ucieklak K, Koj S, Niedziela T.
europepmc +2 more sources
Positivity analysis of Bordetella spp. and SARS-CoV-2 coinfections in clinical samples from Colombia, 2021–2022 [PDF]
Microbiology SpectrumPertussis and COVID-19 are respiratory diseases with similar clinical manifestations, complicating diagnosis. In Colombia, pertussis surveillance is based on probable cases confirmed through laboratory detection of Bordetella pertussis.
Valentina Bonilla-Bravo +11 more
doaj +2 more sources
Genome-based prediction of cross-protective, HLA-DR-presented epitopes as putative vaccine antigens for multiple Bordetella species [PDF]
Microbiology SpectrumAcellular pertussis vaccines protect against severe pertussis, but vaccine-induced immunity wanes over time. Prior animal studies showed that T-cell responses are integral to long-lasting immunity.
Muktha S. Natrajan +9 more
doaj +2 more sources
Bordetella holmesii endocarditis in a patient with systemic lupus erythematous treated with immunosuppressive agents [PDF]
Journal of chemotherapy, 2012 Barbieri, Ramona +6 more
core +2 more sources
Standard Operating Procedure for Real-time PCR Detection and Identification of Bordetella pertussis, B. parapertussis, and B. holmesii using AB7500 [PDF]
, 2018 The appropriate use of clinically accurate diagnostic tests is essential for the detection of pertussis, a poorly controlled vaccine-preventable disease. The purpose of this study was to estimate the sensitivity and specificity of different diagnostic criteria including culture, multi-target polymerase chain reaction (PCR), anti-pertussis toxin IgG ...
Adria D Lee
semanticscholar +2 more sources