Results 71 to 80 of about 12,358,808 (165)
B. parapertussis is killed by NETs.
Neutrophils were incubated with PMA (100 nM) for 1 h at 37°C to induce NETs release. Next, neutrophils were treated with cytochalasinD (100 μg/ml; CytD), MNase I (500 mU/ml), or a combination of both for 30 min at 37°C.
Emilia Scharrig (599847) +4 more
core +1 more source
Saliva and Anterior Nasal Samples for Detecting Respiratory Viruses in Children
ABSTRACT For detecting respiratory viruses, a nasopharyngeal swab sample for polymerase chain reaction (PCR) assays has been the benchmark. Less discomfort related to sampling is requested especially for children, and thus other sample types have been suggested.
Anu Haaramo +5 more
wiley +1 more source
ABSTRACT Background Human metapneumovirus (hMPV) has been increasingly recognized as a major contributor to respiratory infections in all age groups. Owing to its recent discovery, available data on the burden of hMPV in adults are still scant and heterogeneous.
Alexander Domnich +6 more
wiley +1 more source
Impact of nutrient medium composition on protein profiling of Bordetella spp. [PDF]
Objective. To perform comparative analysis of Bordetella protein profiling data and their dependence on various nutrient media. Materials and Methods. This prospective open single-center study, the protein profiles of B. pertussis, B.
Vidmanova M.V. +5 more
doaj +1 more source
Naïve serum (white) or (A) aP, (B) wP, (C) wPP-induced serum (grey)-opsonized GFP-expressing bacteria were incubated with freshly isolated human peripheral blood PMNs. Representative histograms of flow cytometry analysis of these cells are shown.
Xuqing Zhang (144598) +2 more
core +1 more source
Acellular pertussis vaccines protect against severe pertussis, but vaccine-induced immunity wanes over time. Prior animal studies showed that T-cell responses are integral to long-lasting immunity.
Muktha S. Natrajan +9 more
doaj +1 more source
The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis [PDF]
Despite excellent vaccine coverage in developed countries, whooping cough is a reemerging disease that can be caused by two closely related pathogens, Bordetella pertussis and B. parapertussis.
Rodríguez, María Eugenia +4 more
core
O-antigen decreases the opsonization of B. parapertussis by B. pertussis vaccine-induced antibodies.
Representative histograms show flow cytometric analysis of indicated bacteria opsonized with naïve serum (white) or (A) aP, (B) wP, (C) wPP-induced serum (grey) and stained with RPE-labeled goat F(ab)2 fragments of anti-mouse IgG.
Xuqing Zhang (144598) +2 more
core +1 more source
"Direct detenction of B.Pertussis and B. Parapertussis: comparison of PCR and culture"
We evaluated the diagnostic performance of a genomic DNA amplification method for Bordetella pertussis and Bordetella parapertussis compared with culture isolation. Aliquots from B. pertussis and B.
ROLLO MC +5 more
core
A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B.
Farrell, D. J. +2 more
core +1 more source

