Metabolic Engineering of Bacillus methanolicus MGA3 for Production of Acetoin [PDF]
Being a pure, inexpensive, non-food chemical that can be sustainably produced, methanol has attracted great interest as a carbon feedstock for microbial production of commodity/specialty chemicals. In this thesis, methanol-based production of acetoin was
Chan, Dennis Tin Chat
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Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria
Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy
Tami L. McTaggart +7 more
doaj +1 more source
Composition analysis and minimal treatments to solubilize 6 polysaccharides from the brown seaweed Laminaria digitata 7 for microbial growth of thermophiles [PDF]
Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems ...
Aasen, Inga Marie +4 more
core +1 more source
Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins.
Eivind B. Drejer +3 more
doaj +1 more source
Structural classification of biotin carboxyl carrier proteins [PDF]
We gathered primary and tertiary structures of acyl-CoA carboxylases from public databases, and established that members of their biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) domains occur in one family each and that members of ...
Cantu, David +4 more
core +3 more sources
Methanol, a sustainable and abundant one‐carbon feedstock, can be utilized by natural methylotrophic yeasts to synthesize valuable chemicals. However, methanol‐based manufacturing with these yeasts faces several challenges, including low assimilation efficiency, carbon loss, and methanol toxicity. This review summarizes and discusses recent engineering
Shuxian Wang +4 more
wiley +1 more source
In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold +11 more
wiley +1 more source
Process Cascade for the Production of Green Polymers from CO2 and Electric Energy
A novel process cascade is introduced that combines the advantages of electrochemical CO2 conversion as CCU approach with the unrivaled synthetic potential of industrial biotechnology: CO2 is electrocatalytically reduced to formic acid which serves as substrate for the metabolically engineered bacterium Methylorubrum extorquens that prduces l‐lysine as
Jonathan Thomas Fabarius +18 more
wiley +1 more source
CRISPR-Cas9-driven genome editing in Bacillus methanolicus MGA3
Bacillus methanolicus is a thermophilic methylotroph that uses methanol as a sustainable feedstock in biotechnological industry. Here, we developed the CRISPR-Cas9 genome editing tool for B. methanolicus MGA3.
May L. K. Khider +9 more
doaj +1 more source
Establishing a Bacillus methanolicus - Corynebacterium glutamicum consortium towards the sustainable production of amino acids [PDF]
Kjemikalier med høy kommersiell verdi, som aminosyrer, produseres hovedsakelig ved fermentering av plantebaserte produkter i dagens industrielle bioteknologi.
Kambe, Kristin Heggebø
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