Results 51 to 60 of about 4,853,662 (342)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Discovery of the widespread site-specific single-stranded nuclease family Ssn

Nature Communications
Site-specific endonucleases that exclusively cut single-stranded DNA have hitherto never been described and constitute a barrier to the development of ssDNA-based technologies.
Martin Chenal, Alex Rivera-Millot, Luke B. Harrison, Ahmed S. Khairalla, Cecilia Nieves, Ève Bernet, Mansoore Esmaili, Manel Belkhir, Jonathan Perreault, Frédéric J. Veyrier   +9 more
doaj   +1 more source

Continuous watermelon cropping impairs plant growth by modifying soil biochemistry and rhizosphere microbial communities

Frontiers in Microbiology
Continuous watermelon cropping leads to increases in soil-borne diseases, which negatively affect plant growth. We investigated the impact of continuous watermelon cropping on soil biochemical properties, enzyme activities, microbial biomass, occurrence ...
HuiFang Lv, HuiFang Lv, Rui Sang, LingLing Huang, YueChen Long, DeCong Xu, MingXia Wang, QiAn Zhang, Li Jia, QiangQiang Ding, CongSheng Yan, HuanXin Zhang   +11 more
doaj   +1 more source

Variation in bacterial populations in time and space [PDF]

, 1977
Obtaining a reliable estimate of the bacterial population is one of the main problems facing the bacterial ecologist. The author discusses the various methods available and concludes that the observed variability in bacterial populations depends on the ...
Jones, J.G.
core  

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Soil depth exerts greater effect on bacterial community than spatial structure in Longmenshan fault zone

Applied and Environmental Microbiology
The diversity patterns and drivers of soil microbial communities across spatial distances have been extensively investigated over the recent years.
Peng Jiang, Xin Wan, Mingxuan Che, Lihuan Li, Mingxue Liu   +4 more
doaj   +1 more source

Method of detecting and counting bacteria in body fluids [PDF]

, 1973
A novel method is reported for determining bacterial levels in urine samples, which method depends on the quantitative determination of bacterial adenosine triphosphate (ATP) in the presence of non-bacterial ATP.
Chappelle, E. W., Picciolo, G. L.
core   +1 more source

Interkingdom interactions on the denture surface: implications for oral hygiene [PDF]

, 2019
Background: Evidence to support the role of Candida species in oral disease is limited. Often considered a commensal, this opportunistic yeast has been shown to play a role in denture related disease, though whether it is an active participant or ...
Alalwan, Allonsius, Beyer, Caporaso, Cho, Coco, Delaney, Gendreau, Hannah, Janus, Kean, Kean, Kong, Kozich, Kraneveld, Locker, Love, Mantzourani, Martori, Matsuda, McMurdie, Nunes, O'Donnell, O'Donnell, O'Donnell, O'Donnell, Offenbacher, Peric, Peters, Rajendran, Ramage, Ramage, Ramage, Sachdeo, Sherry, Sherry, Shi, Sztukowska, Teles, Yitzhaki   +39 more
core   +2 more sources

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Comprehensive Evaluation of the MBT STAR-BL Module for Simultaneous Bacterial Identification and β-Lactamase-Mediated Resistance Detection in Gram-Negative Rods from Cultured Isolates and Positive Blood Cultures

Frontiers in Microbiology, 2018
Objective: This study evaluated the capability of a MALDI Biotyper system equipped with the newly introduced MBT STAR-BL module to simultaneously perform species identification and β-lactamase-mediated resistance detection in bacteremia -causing bacteria
Annie W. T. Lee, Johnson K. S. Lam, Ricky K. W. Lam, Wan H. Ng, Ella N. L. Lee, Vicky T. Y. Lee, Po P. Sze, Rahim Rajwani, Kitty S. C. Fung, Wing K. To, Rodney A. Lee, Dominic N. C. Tsang, Gilman K. H. Siu   +12 more
doaj   +1 more source