Results 31 to 40 of about 3,675,539 (309)
Protocol Exchange, 2018 Here we present a modified version of the Marmur procedure (Marmur, 1961) for extraction, isolation and purification of bacterial DNA. The protocol is effective in providing large amounts of high quality and high molecular weight genomic DNA from Gram ...
F. Salvà-Serra +6 more
semanticscholar +1 more source
An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents. [PDF]
PLoS ONE, 2011 BACKGROUND: Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria.
Shy-Shin Chang +3 more
doaj +1 more source
Mobility of bacterial protein Hfq on dsDNA; Role of C-terminus mediated transient binding [PDF]
arXiv, 2021 The mobility of protein is fundamental in the machinery of life. Here, we have investigated the effect of DNA binding in conjunction with DNA internal motion of the bacterial Hfq master regulator devoid of its amyloid C-terminus domain. Hfq is one of the most abundant nucleoid associated proteins that shape the bacterial chromosome and is involved in ...
arxiv
Bacterial nucleoid: Interplay of DNA demixing and supercoiling [PDF]
Biophysical Journal 118 (2020) 2141-2150, 2019 This work addresses the question of the interplay of DNA demixing and supercoiling in bacterial cells. Demixing of DNA from other globular macromolecules results from the overall repulsion between all components of the system and leads to the formation of the nucleoid, which is the region of the cell that contains the genomic DNA in a rather compact ...
arxiv +1 more source
New Microbes and New Infections, EarlyView., 2014 Abstract We describe a case of fungal keratitis due to Beauveria bassiana in a farmer with Fuchs' dystrophy, treated with amphotericin B. Surgery with penetrating keratoplasty was necessary to resolve the lesions. Susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive ...
M. Ligozzi +8 more
wiley +1 more source
A model of H-NS mediated compaction of bacterial DNA [PDF]
Biophysical Journal 104 (2013) 1615-1622, 2013 The Histone-like Nucleoid Structuring protein (H-NS) is a nucleoid-associated protein, which is involved in both gene regulation and DNA compaction. H-NS can bind to DNA in two different ways: in trans, by binding to two separate DNA duplexes, or in cis, by binding to different sites on the same duplex.
arxiv +1 more source
Bacterial DNA promotes Tau aggregation
Scientific Reports, 2019 A hallmark feature of Alzheimer’s disease (AD) and other tauopathies is the misfolding, aggregation and cerebral accumulation of tau deposits. Compelling evidence indicates that misfolded tau aggregates are neurotoxic, producing synaptic loss and ...
G. Tetz +5 more
semanticscholar +1 more source
Q fever outbreak in the terraced vineyards of Lavaux, Switzerland
New Microbes and New Infections, EarlyView., 2014 Abstract Coxiella burnetii infection (Q fever) is a widespread zoonosis with low endemicity in Switzerland, therefore no mandatory public report was required. A cluster of initially ten human cases of acute Q fever infections characterized by prolonged fever, asthenia and mild hepatitis occurred in 2012 in the terraced vineyard of Lavaux ...
C. Bellini +9 more
wiley +1 more source
Natural History of DNA-Dependent DNA Polymerases: Multiple Pathways to the Origins of DNA
Viruses, 2023 One of the major evolutionary transitions that led to DNA replacing RNA as the primary informational molecule in biological systems is still the subject of an intense debate in the scientific community.
Sávio Torres de Farias +3 more
doaj +1 more source
A model capturing novel strand symmetries in bacterial DNA [PDF]
Biochemical and Biophysical Research Communications, Volume 410,
Issue 4, 15 July 2011, Pages 823-828, ISSN 0006-291X, 2014 Chargaff's second parity rule for short oligonucleotides states that the frequency of any short nucleotide sequence on a strand is approximately equal to the frequency of its reverse complement on the same strand. Recent studies have shown that, with the exception of organellar DNA, this parity rule generally holds for double stranded DNA genomes and ...
arxiv +1 more source