Results 81 to 90 of about 1,273,913 (331)

DNA vaccines for targeting bacterial infections [PDF]

Expert Review of Vaccines, 2010
DNA vaccination has been of great interest since its discovery in the 1990s due to its ability to elicit both humoral and cellular immune responses. DNA vaccines consist of a DNA plasmid containing a transgene that encodes the sequence of a target protein from a pathogen under the control of a eukaryotic promoter.
David B. Weiner, Omkar U. Kawalekar, Karuppiah Muthumani, Devon J. Shedlock, Mariana Ingolotti   +4 more
openaire   +3 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Mechanisms of bacterial DNA replication restart [PDF]

Nucleic Acids Research, 2017
Multi-protein DNA replication complexes called replisomes perform the essential process of copying cellular genetic information prior to cell division. Under ideal conditions, replisomes dissociate only after the entire genome has been duplicated. However, DNA replication rarely occurs without interruptions that can dislodge replisomes from DNA.
James L. Keck, Basudeb Bhattacharyya, Basudeb Bhattacharyya, Tricia A. Windgassen, Sarah R. Wessel, Sarah R. Wessel   +5 more
openaire   +3 more sources

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Effect of bacterial DNA enrichment on detection and quantification of bacteria in an infected tissue model by metagenomic next-generation sequencing

ISME Communications, 2022
Before implementing metagenomic next-generation sequencing (mNGS) in the routine diagnostic laboratory, several challenges need to be resolved. To address strengths and limitations of mNGS in bacterial detection and quantification in samples with ...
Vladimir Lazarevic, Nadia Gaïa, Myriam Girard, Florian Mauffrey, Etienne Ruppé, Jacques Schrenzel   +5 more
doaj   +1 more source

The process of infection with bacteriophage phiX174, XXI. Replication and fate of the replicative form [PDF]

, 1968
Previous publications have described the formation of a double-stranded DNA intermediate(1) (replicative form, RF) during the replication of bacteriophage phiX174, the semiconservative replication at a bacterial "site"(2) of that RF containing the ...
Knippers, Rolf, Komano, Tohru, Sinsheimer, Robert L.   +2 more
core  

Structurally specific thermal fluctuations identify functional sites for DNA transcription

, 2003
We report results showing that thermally-induced openings of double stranded DNA coincide with the location of functionally relevant sites for transcription.
A Usheva, A. R Bishop, C. H Choi, Concino M. F., Fields B. N., Frauenfelder H., G Kalosakas, K. Ø Rasmussen, Stryer L.   +8 more
core   +1 more source

Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling. [PDF]

, 2015
DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is essential for DNA replication and gene regulation. However, the density of supercoiling in vivo is circa twice smaller than in deproteinized DNA molecules isolated ...
Benedetti, F., Burnier, Y., Dietler, G., Dorier, J., Japaridze, A., Kwapich, R., Racko, D., Stasiak, A.   +7 more
core   +2 more sources

Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]

, 2020
Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J., Deckers, Marie, Deforce, Dieter, Denayer, Sarah, Fraiture, Marie-Alice, Heyndrickx, Marc, Roosens, Nancy H. C., Vanneste, Kevin, Winand, Raf   +8 more
core   +2 more sources