Unlimited cooperativity ofBetatectivirusSSB, a novel DNA binding protein related to an atypical group of SSBs from protein-primed replicating bacterial viruses [PDF]
, 2021 Ana Lechuga +3 more
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SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Intravesicle Isothermal DNA Replication
BMC Research Notes, 2011 Background Bacterial and viral DNA replication was previously reconstituted in vitro from component parts 1234. Significant advances in building minimal cell-like structures also have been made recently 567.
Ross Lindsey A +4 more
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Programming the surface of probiotics to mitigate bacterial toxin-mediated DNA damage [PDF]
, 2022 Songqi Zhang
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Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
Multiple gene sequence analysis using genes of the bacterial DNA repair pathway
Brazilian Archives of Biology and Technology, 2015 The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways.
Miguel Rotelok Neto +6 more
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Hyperchromicity and Strand Separation in Bacterial DNA
Biophysical Journal, 1962 Studies of the per cent of strand separation of N(14)-N(15) hybrid coli DNA heated to various temperatures in formaldehyde have shown that the process of strand separation is a function of temperature and formaldehyde concentration and is directly related to the measured hyperchromicity.
Freifelder, David, Davison, Peter F.
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Bacterial isolation and genomic DNA extraction of Mycobacterium avium from pig lymph nodes v1
, 2021 Tetsuya Komatsu
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In Silico Studies on Functional Significance of Multiple Binding Configurations of Bacterial Nucleoid Associated Protein-DNA Assemblies [PDF]
, 2020 Min‐Yeh Tsai +3 more
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