Results 31 to 40 of about 237 (185)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Molecular Methods for Bacterial Strain Typing

Medical Laboratory Journal, 2016
Typing of bacteria is an important part of epidemiological studies on nosocomial infections. Bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. In many
Leili Shokoohizadeh
doaj  

Molecular typing of uropathogenic isolated from Korean children with urinary tract infection [PDF]

Korean Journal of Pediatrics, 2015
PurposeWe investigated the molecular types of uropathogenic Escherichia coli (UPEC) by using conventional phylogrouping, multilocus sequence typing (MLST), and fimH genotyping.MethodsSamples of patients younger than 18 years of age were collected from ...
Ki Wook Yun, Do Soo Kim, Wonyong Kim, In Seok Lim   +3 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

MYCOBACTERIUM AVIUM AS AN ACTUAL PATHOGEN OF HUMAN MYCOBACTERIOSIS

Инфекция и иммунитет, 2014
. Mycobacteriosis is an infectious disease of animals and humans caused by non-tuberculosis mycobacteria including M. avium complex. Despite the fact that the transmission of M.
D. A. Starkova
doaj   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Direct and indirect methods of determining DNA nucleotide sequences in microorganisms

Биопрепараты: Профилактика, диагностика, лечение, 2018
Typing of bacterial populations and identification of microorganisms are the overriding priorities in the field of prophylaxis, diagnosis and treatment of bacterial infections. Over the recent two decades rapidly developing approaches to molecular typing
R. A. Volkova, E. S. Skolotneva, E. V. Elbert, E. D. Mytsa, V. A. Merkulov, V. P. Bondarev, I. V. Borisevich   +6 more
doaj  

Caracterización de dos brotes de fiebre tifoidea en Apartadó, Antioquia, 2005

Biomédica: revista del Instituto Nacional de Salud, 2007
Introducción. La caracterización de los brotes de fiebre tifoidea es importante epidemiológicamente, debido a que esto permite la búsqueda de la fuente y el desarrollo de medidas de control. Objetivo. Describir un brote de fiebre tifoidea en el municipio
Nora María Cardona-Castro, Miryan Margot Sánchez-Jiménez, Luz Yaned Usuga-Silva, Margarita Arboleda-Naranjo, Eliana Garzón, Aminta Vélez, Magdalena Wiesner, Nélida Muñoz, Clara Inés Agudelo   +8 more
doaj   +1 more source

Development of a Trypanosoma cruzi strain typing assay using MS2 peptide spectral libraries (Tc-STAMS2). [PDF]

PLoS Neglected Tropical Diseases, 2018
Chagas disease also known as American trypanosomiasis is caused by the protozoan Trypanosoma cruzi. Over the last 30 years, Chagas disease has expanded from a neglected parasitic infection of the rural population to an urbanized chronic disease, becoming
Gilberto Santos de Oliveira, Rebeca Kawahara, Livia Rosa-Fernandes, Simon Ngao Mule, Carla Cristi Avila, Marta M G Teixeira, Martin R Larsen, Giuseppe Palmisano   +7 more
doaj   +1 more source