Identification of lectin counter-receptors on cell membranes by proximity labelling [PDF]
, 2017 Lectin-glycan interactions play important roles in many biological systems, but the nature of glycoprotein counter-receptors expressed on cell membranes is often poorly understood.
Crocker, Paul R. +2 more
core +2 more sources
An Artificial Molecular Pump Powered by Light
Angewandte Chemie, EarlyView.A minimal rotaxane‐based supramolecular system functions as a light‐powered molecular pump, capturing macrocycles from solution and concentrating them within an intramolecular compartment. Repeated azobenzene photoisomerization modulates the binding and kinetic barriers of threading and shuttling, generating a photon‐driven energy ratchet that sustains
Federico Nicoli +7 more
wiley +2 more sources
An Atomic Force Microscope with Dual Actuation Capability for Biomolecular Experiments [PDF]
, 2016 We report a modular atomic force microscope (AFM) design for biomolecular experiments. The AFM head uses readily available components and incorporates deflection-based optics and a piezotube-based cantilever actuator.
Ergeneman, Olgaç +8 more
core +1 more source
Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula [PDF]
, 2015 Functional genomics of diatom-dominated communities from the Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the ...
Agusti, Susana +8 more
core +1 more source
Quantifying the Relative Importance of Phylogeny and Environmental Preferences As Drivers of Gene Content in Prokaryotic Microorganisms [PDF]
, 2016 Two complementary forces shape microbial genomes: vertical inheritance of genes by phylogenetic descent, and acquisition of new genes related to adaptation to particular habitats and lifestyles.
Carlos ePedrós-Alió +4 more
core +2 more sources
De Novo sequences of Haloquadratum walsbyi from Lake Tyrrell, Australia, reveal a aariable genomic landscape [PDF]
, 2015 Hypersaline systems near salt saturation levels represent an extreme environment, in which organisms grow and survive near the limits of life. One of the abundant members of the microbial communities in hypersaline systems is the square archaeon ...
Allen, Eric E +6 more
core +2 more sources
Plant High-Affinity Potassium (HKT) transporters involved in salinity tolerance: structural insights to probe differences in ion selectivity [PDF]
, 2013 High-affinity Potassium Transporters (HKTs) belong to an important class of integral membrane proteins (IMPs) that facilitate cation transport across the plasma membranes of plant cells.
Alberts +95 more
core +3 more sources
Directed Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a Synthetic Chromophore [PDF]
, 2017 By engineering a microbial rhodopsin, Archaerhodopsin-3 (Arch), to bind a synthetic chromophore, merocyanine retinal, in place of the natural chromophore all-trans-retinal (ATR), we generated a protein with exceptionally bright and unprecedentedly red ...
Arnold, Frances H. +11 more
core +2 more sources
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
On the relationship between thermal stability and catalytic power of enzymes [PDF]
, 2007 The possible relationship between the thermal stability and the catalytic power of enzymes is of great current interest. In particular, it has been suggested that thermophilic or hyperthermophilic (Tm) enzymes have ...
Liu, Hanbin +3 more
core +1 more source