Results 31 to 40 of about 1,270 (179)

Damage‐limited resolution for X‐ray and electron microscopy of organic specimens

Acta Crystallographica Section D, Volume 82, Issue 5, Page 471-483, May 2026.
Analytical expressions for the damage‐limited resolution are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens. The findings will guide future microscopy and instrument design.Analytical expressions for the damage‐limited resolution (DLR) are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens ...
Ray F. Egerton, Colin Nave
wiley   +1 more source

Mapping the effects of specific radiation damage and solvent radiolysis in buffers and crystals with online UV–Vis absorption spectroscopy

Acta Crystallographica Section D, Volume 82, Issue 5, Page 504-515, May 2026.
Radiation‐induced changes during X‐ray crystallographic data collection can compromise concurrent spectroscopic studies. Suitable protocols to detect and identify spectral artefacts from crystallization buffers and cryoprotectants are essential for the reliable analysis of chromophoric ligands in macromolecules.Online UV–Vis absorption spectroscopy is ...
Jack Stubbs, Nicolas Caramello, Matthew J. Rodrigues, Sylvain Engilberge, Eric Mathieu, Samuel L. Rose, Gwyndaf Evans, Antoine Royant, Ivo Tews   +8 more
wiley   +1 more source

Chromophore equilibria in bacteriorhodopsin

Biophysical Journal, 1979
An investigation of the dark equilibria between different chromophores of bacteriorhodopsin (BR) and studies of the kinetics of their interconversion and photochemical activity have led to the following conclusions. (a) A component of the 605-nm chromophore of BR decays in the millisecond range and is likely to be identical to the intermediate O of the
Fischer, U., Oesterhelt, D.
openaire   +2 more sources

The Conformational Switches of a Bacterial Light‐Driven Sodium Pump Characterized by Time‐Resolved Resonance Raman Spectroscopy

ChemPhysChem, Volume 27, Issue 7, 14 April 2026.
The microbial retinal protein KR2 (Krokinobacter eikastus) is a light‐driven outward Na+ pump, which is coupled to the photocycle of the chromophore. Using time‐resolved continuous‐wave pump‐probe resonance Raman spectroscopy, the operational switches for ion transport are identified, which are the transitions to a specific Schiff base sub‐state and ...
Anna Lena Schäfer, Arita Silapetere, Peter Hegemann, Katrina T. Forest, Uwe Kuhlmann, Peter Hildebrandt   +5 more
wiley   +1 more source

Chromophore motion during the bacteriorhodopsin photocycle: polarized absorption spectroscopy of bacteriorhodopsin and its M-state in bacteriorhodopsin crystals. [PDF]

The EMBO Journal, 1991
The three-dimensional crystallization of bacteriorhodopsin was systematically investigated and the needle-shaped crystal form analysed. In these crystals the M-intermediate forms 10 times faster and decays 15 times more slowly than in purple membranes. Polarized absorption spectra of the crystals were measured in the dark and light adapted states.
Schertler, G., Lozier, R., Michel, H. ; https://orcid.org/0000-0003-4351-7846, Oesterhelt, D.   +3 more
openaire   +3 more sources

BIOORGANIC CHEMISTRY OF THE PURPLE MEMBRANE OF HALOBACTERIUM-HALOBIUM - CHROMOPHORE AND APOPROTEIN MODIFIED BACTERIORHODOPSINS

, 1988
Iodophenyl and anthryl retinal analogues have been synthesized. Thetrans-isomers have been isolated and purified by high pressure liquid chromatography.
ROY, M, KAPIL, M, SINGH, AK, SONAR, S
core   +2 more sources

Carbon Contacts to Proteins Enable Robust, Biocompatible Electronic Junctions with Near‐Activation‐less Conduction Down to 10 K

Advanced Functional Materials, Volume 36, Issue 31, 16 April 2026.
A robust solid‐state protein junction with a semi‐transparent eC/Au electrode allows photoexcitation of the bacterio‐rhodopsin, bR layer, to isomerize the bR retinal. The resulting photo‐response shows the protein is functional in the solid‐state junction.
Shailendra K. Saxena, Sudipta Bera, Tatyana Bendikov, Israel Pecht, Mordechai Sheves, David Cahen   +5 more
wiley   +1 more source

A New Era for Using Natural Pigments: The Case of the C50 Carotenoid Called Bacterioruberin

Biotechnology and Applied Biochemistry, Volume 73, Issue 2, Page 913-926, April 2026.
ABSTRACT Haloarchaea are extremophilic microorganisms belonging to the Archaea domain that require high salt concentrations to live, thus inhabiting ecosystems like salty ponds, salty marshes, or extremely salty lagoons. They are more abundant and widely distributed worldwide than initially expected.
Micaela Giani, Elena Valdés, José María Miralles‐Robledillo, Guillermo Martínez, Carmen Pire, Rosa María Martínez‐Espinosa   +5 more
wiley   +1 more source

Photo‐Modulated Proton Transport in Merocyanine Metastable‐State Photoacid Based Polymers

Small, Volume 22, Issue 23, 22 April 2026.
The covalent incorporation of merocyanine metastable‐state photoacid into soft polymers imparts conductivity to otherwise insulating matrices, allowing light‐triggered control of charge and structure, offering a new platform for photo‐responsive, ion‐conducting materials.
Gianni Pacella, Mira Kim, Rachael Hannah, Ryan C. Chiechi, Shirin Faraji, Giuseppe Portale   +5 more
wiley   +1 more source

Precise Capture of Membrane Proteins Using DNA‐Origami‐Constrained Nanodiscs

Small Structures, Volume 7, Issue 4, April 2026.
A designed DNA origami structure ensures manipulates and inserts an individual membrane protein in a single, captured nanodisc. Membrane proteins play a key role as cellular gatekeepers, regulating the flow of information and material across lipid membranes.
Piotr Stepien, Gerrit Wilkens, Sylwia Swiatek, Manuel Yusef Robles, Anna Swietlikowska, Sarah Hutchings, Dmitry Ghilarov, Jonathan G. Heddle   +7 more
wiley   +1 more source