Damage‐limited resolution for X‐ray and electron microscopy of organic specimens
Analytical expressions for the damage‐limited resolution are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens. The findings will guide future microscopy and instrument design.Analytical expressions for the damage‐limited resolution (DLR) are developed and applied to X‐ray and electron imaging of beam‐sensitive specimens ...
Ray F. Egerton, Colin Nave
wiley +1 more source
Radiation‐induced changes during X‐ray crystallographic data collection can compromise concurrent spectroscopic studies. Suitable protocols to detect and identify spectral artefacts from crystallization buffers and cryoprotectants are essential for the reliable analysis of chromophoric ligands in macromolecules.Online UV–Vis absorption spectroscopy is ...
Jack Stubbs +8 more
wiley +1 more source
The physics of pulling polyproteins: a review of single molecule force spectroscopy using the AFM to study protein unfolding. [PDF]
One of the most exciting developments in the field of biological physics in recent years is the ability to manipulate single molecules and probe their properties and function.
Dougan, L, Hughes, ML
core +1 more source
The microbial retinal protein KR2 (Krokinobacter eikastus) is a light‐driven outward Na+ pump, which is coupled to the photocycle of the chromophore. Using time‐resolved continuous‐wave pump‐probe resonance Raman spectroscopy, the operational switches for ion transport are identified, which are the transitions to a specific Schiff base sub‐state and ...
Anna Lena Schäfer +5 more
wiley +1 more source
A robust solid‐state protein junction with a semi‐transparent eC/Au electrode allows photoexcitation of the bacterio‐rhodopsin, bR layer, to isomerize the bR retinal. The resulting photo‐response shows the protein is functional in the solid‐state junction.
Shailendra K. Saxena +5 more
wiley +1 more source
Ultragyors lineáris és nemlineáris optikai folyamatok makromolekulákon = Ultrafast linear and nonlinear optical processes on macromolecules [PDF]
Mérőrendszerünkhöz új tükörkészletet és diszperzió szabályozó egységet fejlesztettünk ki, széles hullámhossz tartományban lehetővé téve (akár 40 fs-nál rövidebb) stabil fényimpulzusok keltését.
Bányász, Ákos +9 more
core
A New Era for Using Natural Pigments: The Case of the C50 Carotenoid Called Bacterioruberin
ABSTRACT Haloarchaea are extremophilic microorganisms belonging to the Archaea domain that require high salt concentrations to live, thus inhabiting ecosystems like salty ponds, salty marshes, or extremely salty lagoons. They are more abundant and widely distributed worldwide than initially expected.
Micaela Giani +5 more
wiley +1 more source
The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity [PDF]
BACKGROUND: The square halophilic archaeon Haloquadratum walsbyi dominates NaCl-saturated and MgCl(2 )enriched aquatic ecosystems, which imposes a serious desiccation stress, caused by the extremely low water activity.
Bolhuis, Henk +7 more
core +2 more sources
Photo‐Modulated Proton Transport in Merocyanine Metastable‐State Photoacid Based Polymers
The covalent incorporation of merocyanine metastable‐state photoacid into soft polymers imparts conductivity to otherwise insulating matrices, allowing light‐triggered control of charge and structure, offering a new platform for photo‐responsive, ion‐conducting materials.
Gianni Pacella +5 more
wiley +1 more source
Properties of Asp212—-Asn bacteriorhodopsin suggest that Asp212 and Asp85 both participate in a counterion and proton acceptor complex near the Schiff base [PDF]
The gene coding for bacteriorhodopsin was modified in vitro to replace Asp212 with asparagine and expressed in Halobacterium halobium. X-ray diffraction measurements showed that the major lattice dimension of purple membrane containing the mutated ...
Chang, M +6 more
core +1 more source

