Results 41 to 50 of about 10,209 (169)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus.

The Journal of Antibiotics, 1983
The susceptibility of 80 Bacteroides fragilis group strains isolated from clinical specimens to beta-lactam antibiotics was investigated by agar dilution method. Twenty strains showed high resistance to the antibiotics. The resistance level of the isolates to cephaloridine was related to the amount of beta-lactamase activity (cephalosporinase; CSase ...
K, Sato, Y, Matsuura, K, Miyata, M, Inoue, S, Mitsuhashi   +4 more
openaire   +3 more sources

Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces [PDF]

Applied and Environmental Microbiology, 2005
ABSTRACT A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron , generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples.
C Andrew, Carson, Jessica M, Christiansen, Helen, Yampara-Iquise, Verel W, Benson, Claire, Baffaut, Jerri V, Davis, Robert R, Broz, William B, Kurtz, Wendi M, Rogers, William H, Fales   +9 more
openaire   +2 more sources

Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles

Journal of Extracellular Vesicles
Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood.
Rokas Juodeikis, Carlo Martins, Gerhard Saalbach, Jake Richardson, Todor Koev, Dave J. Baker, Marianne Defernez, Martin Warren, Simon R. Carding   +8 more
doaj   +1 more source

Bacteroides thetaiotaomicron Fosters the Growth of Butyrate-Producing Anaerostipes caccae in the Presence of Lactose and Total Human Milk Carbohydrates

Microorganisms, 2020
The development of infant gut microbiota is strongly influenced by nutrition. Human milk oligosaccharides (HMOSs) in breast milk selectively promote the growth of glycan-degrading microbes, which lays the basis of the microbial network. In this study, we
Loo Wee Chia, Marko Mank, Bernadet Blijenberg, Steven Aalvink, Roger S. Bongers, Bernd Stahl, Jan Knol, Clara Belzer   +7 more
doaj   +1 more source

Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species

Frontiers in Microbiology, 2022
IntroductionGlycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
Annelieke Overbeeke, Annelieke Overbeeke, Bela Hausmann, Bela Hausmann, Georgi Nikolov, Fatima C. Pereira, Craig W. Herbold, David Berry, David Berry   +8 more
doaj   +1 more source

Oligosaccharides With Defined Glycosidic Bonds Shape Gut Microbial Succession and Metabolism Via Bond‐Specific Microbial Responders

Advanced Science, EarlyView.
Oligosaccharides with defined glycosidic bonds drive divergent gut microbial succession and shape unique metabolic profiles. Absolute time‐series profiling with Bayesian generalized Lotka–Volterra modeling identifies bond‐specific microbial responders, while monoculture validation and metaproteomics reveal linkage‐matched transport and cleavage modules.
Xiaoxuan Lu, Jiaqi Zou, Geng Han, Mengyao Zhao, Ting Luo, Xiangru Feng, Liangliang Zhu, Yijia Chen, Xiaoguo Ji, Jiayang Jin, Liming Zhao   +10 more
wiley   +1 more source

Characterization of the role of glycine lipids in Bacteroides thetaiotaomicron [PDF]

, 2018
Abstract Acylated amino acids function as important components of the cellular membrane in some bacteria. Biosynthesis is initiated by the N-acylation of the amino acid and this is followed by subsequent O-acylation of the acylated molecule resulting in the production of the mature diacylated amino acid lipid. In this
Lynch, Alli, Tammireddy, Seshu R., Doherty, Mary K., Whitfield, Phillip D., Clarke, David J.   +4 more
openaire   +1 more source

Transglycosylation Properties of a Novel α-1,4-Glucanotransferase from Bacteroides thetaiotaomicron and Its Application in Developing an α-Glucosidase-Specific Inhibitor

Journal of Chemistry, 2018
In this study, α-glucanotransferase from Bacteroides thetaiotaomicron was expressed in Escherichia coli and characterized. Conserved amino-acid sequence alignment showed that Bacteroides thetaiotaomicron α-glucanotransferase (BtαGTase) belongs to the ...
Hye-Jeong Choi, Dam-Seul Ko, Na-Ri Kim, Woo-Jae Choung, Ye-Seul Koo, Da-Woon Jeong, Jae-Hoon Shim   +6 more
doaj   +1 more source

Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic Steatosis

Cell Reports, 2020
Summary: A beneficial gut Bacteroides-folate-liver pathway regulating lipid metabolism is demonstrated. Oral administration of a Ganoderma meroterpene derivative (GMD) ameliorates nonalcoholic hepatic steatosis in the liver of fa/fa rats by reducing ...
Shanshan Qiao, Li Bao, Kai Wang, Shanshan Sun, Mingfang Liao, Chang Liu, Nan Zhou, Ke Ma, Yuwei Zhang, Yihua Chen, Shuang-Jiang Liu, Hongwei Liu   +11 more
doaj   +1 more source