Results 51 to 60 of about 15,598 (206)
Angewandte Chemie, EarlyView.We report plate‐based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin‐active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor identification and allows determination of (sub‐)cellular location. ABSTRACT Sulfated glycans play a central role in human health and influence cell signaling,
Charles W. E. Tomlinson +5 more
wiley +2 more sources
Preferential Packing of Acidic Glycosidases and Proteases into
mBio, 2014 Outer membrane vesicles (OMV) are spherical membranous structures released from the outer membrane (OM) of Gram-negative bacteria. OMV have been proposed to play several different roles during both pathogenesis and symbiosis.
Wael Elhenawy +2 more
doaj +1 more source
Ruminococcal cellulosome systems from rumen to human [PDF]
, 2015 This article is protected by copyright. All rights reserved. The authors appreciate the kind assistance of Miriam Lerner (ImmunArray Ltd. Company, Rehovot, Israel) with experiments involving the MicroGrid II arrayer.
Bayer, Edward A +11 more
core +3 more sources
Induction of chondroitin sulfate lyase activity in Bacteroides thetaiotaomicron [PDF]
Journal of Bacteriology, 1980 Chondroitin sulfate lyase (EC 4.2.2.4) was present constitutively at low levels (0.06 to 0.08 U/mg of protein) in cells of Bacteroides thetaiotaomicron which were growing on glucose or other monosaccharides. When these uninduced bacteria were incubated with chondroitin sulfate A (5 mg/ml), chondroitin sulfate lyase specific activity increased more than
A A, Salyers, S F, Kotarski
openaire +2 more sources
Nature Communications, 2020 Glycosaminoglycans (GAGs) are an important nutrient source for the gut microbiome. Here, the authors characterize the genetic loci that underpins glycosaminoglycan utilization in Bacteroides thetaiotaomicron; providing insights into the metabolism of ...
Didier Ndeh +7 more
doaj +1 more source
GH97 is a new family of glycoside hydrolases, which is related to the α-galactosidase superfamily [PDF]
, 2005 Background As a rule, about 1% of genes in a given genome encode glycoside hydrolases and their homologues. On the basis of sequence similarity they have been grouped into more than ninety GH families during the last 15 years.
Naumoff Daniil G
core +1 more source
Nature Communications, 2020 Bacteroides thetaiotaomicron is a human gut microbe and an emergent model organism. Here, Ryan et al. generate single-nucleotide resolution RNA-seq data for this bacterium and map transcription start sites and noncoding RNAs, one of which modulates ...
Daniel Ryan +4 more
doaj +1 more source
Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library [PDF]
, 2014 BACKGROUND: Bacteroides fragilis is a Gram-negative anaerobe that is normally a human gut commensal; it comprises a small percentage of the gut Bacteroides but is the most frequently isolated Bacteroides from human infections.
Elizabeth L Tenorio +3 more
core +1 more source
Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces [PDF]
Applied and Environmental Microbiology, 2005 ABSTRACT A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron , generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples.
C Andrew, Carson +9 more
openaire +2 more sources
Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii [PDF]
, 2015 ACKNOWLEDGMENTS We acknowledge support from BBSRC grant no. BB/L009951/1, from the Scottish government Food, Land and People program, and from the Society for Applied Microbiology. E.A.B. is supported by a grant (no.
Bayer, Edward A. +11 more
core +4 more sources