Results 11 to 20 of about 99,639 (312)
Pharmaceuticals, 2022 Hydrogel wound dressings are effective in their ability to provide a wound-healing environment but are limited by their ability to promote later stages of revascularization.
Sabrina Schaly +5 more
semanticscholar +1 more source
Baculovirus expression: tackling the complexity challenge [PDF]
, 2013 This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
David Barford +3 more
openalex +5 more sources
Viruses, 2023 Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput and bespoke classical genome manipulation techniques, we set ...
Christopher Nguyen +2 more
doaj +1 more source
Biotechnology Journal, 2020 Despite rapid progress in the field, scalable high‐yield production of adeno‐associated virus (AAV) is still one of the critical bottlenecks the manufacturing sector is facing. The insect cell‐baculovirus expression vector system (IC‐BEVS) has emerged as
P. Joshi +3 more
semanticscholar +1 more source
Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells. [PDF]
PLoS ONE, 2009 The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the ...
Johanna P Laakkonen +10 more
doaj +1 more source
Improving baculovirus recombination [PDF]
Nucleic Acids Research, 2003 Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an
Yuguang, Zhao +2 more
openaire +2 more sources
Significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette. [PDF]
PLoS ONE, 2014 Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus ...
Silvia Gómez-Sebastián +2 more
doaj +1 more source
Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides [PDF]
, 2013 Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer ...
Borio, Cristina Silvia +5 more
core +3 more sources
Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]
, 2016 Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
core +2 more sources
BMC Genomics, 2018 Background Erinnyis ello granulovirus (ErelGV) is a betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops (Manihot esculenta).
A. F. Brito +5 more
doaj +1 more source