Results 231 to 240 of about 82,970 (261)
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2007
The production of a recombinant baculovirus expression vector normally involves mixing infectious virus DNA with a plasmid-based transfer vector and then cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available.
Possee, Robert D., King, Linda A.
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The production of a recombinant baculovirus expression vector normally involves mixing infectious virus DNA with a plasmid-based transfer vector and then cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available.
Possee, Robert D., King, Linda A.
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Baculovirus Phylogeny and Evolution
Current Drug Targets, 2007The family Baculoviridae represents one of the largest and most diverse groups of viruses and a unique model for studying the forces driving the evolution and biodiversity of double-stranded DNA viruses with large genomes. With the advent of comparative genomics, the phylogenetic relationships of baculoviruses have been put on solid bases.
Elisabeth A, Herniou, Johannes A, Jehle
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Transfection with baculovirus DNA
Virology, 1980Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973).
J P, Burand, M D, Summers, G E, Smith
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Manipulation of Baculovirus Vectors
2003The development of expression vectors from baculoviruses has provided molecular biologists with one of the most efficient and effective ways of synthesizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (1,2), this contribution aims to explore the various practical aspects of the system,
M J, Bailey, R D, Possee
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Baculovirus as Vaccine Vectors
Current Gene Therapy, 2010Application of viral vectors derived from human viruses to mediate immune response in animals and humans has been greatly hampered by the problems associated with pre-existing immunity and associated toxicities. Among few non-human viral vectors, baculovirus has now evolved as a novel tool for vaccine vector development.
Madhan, S., Prabakaran, M., Kwang, J.
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Virologica Sinica, 2009
Baculoviruses are used as microbial insecticides, protein expression vectors, epitope display platforms, and most recently as vectors for gene therapy. Understanding the mechanisms that control baculovirus host-range and tissue tropisms are important for assessing their safety and for improving their properties for these biotechnology applications.
Suzanne M. Thiem, Xiao-Wen Cheng
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Baculoviruses are used as microbial insecticides, protein expression vectors, epitope display platforms, and most recently as vectors for gene therapy. Understanding the mechanisms that control baculovirus host-range and tissue tropisms are important for assessing their safety and for improving their properties for these biotechnology applications.
Suzanne M. Thiem, Xiao-Wen Cheng
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Construction of baculovirus recombinants
Cytotechnology, 1996There are three main reasons for wishing to modify baculovirus genomes. The most common arises from the use of baculoviruses to express foreign genes. Baculovirus expression systems frequently give high level expression of proteins with full biological activity.
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Journal of Thermal Biology, 1994
Abstract 1. 1. The effect of different temperature treatments on the thermal stability of three different baculoviruses (1 GV and 2 NPVs) was compared. 2. 2. The tests were carried out using Diatraea saccharalis (sugarcane borer) larvae as a host for the three viruses. 3. 3.
Helena C.T. Ribeiro +1 more
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Abstract 1. 1. The effect of different temperature treatments on the thermal stability of three different baculoviruses (1 GV and 2 NPVs) was compared. 2. 2. The tests were carried out using Diatraea saccharalis (sugarcane borer) larvae as a host for the three viruses. 3. 3.
Helena C.T. Ribeiro +1 more
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Introduction to Baculovirus Molecular Biology
2007The development of baculovirus expression vector systems has accompanied a rapid expansion of our knowledge about the genes, their function, and regulation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in
Kelly, Barbara J. +2 more
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Baculovirus-insect cell interactions
Cytotechnology, 1996Baculovirus interactions with host cells range from the physical interactions that occur during viral binding and entry, to the complex and subtle mechanisms that regulate host gene expression and modify and regulate cellular and organismal physiology and defenses.
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